MRI: Track 1 Acquisition of a Liquid Chromatography-Electrospray Ionization-Quadrupole Time of Flight Mass Spectrometer for Central Michigan University

该奖项由主要研究仪器计划和化学研究仪器计划资助。Choon Young Lee教授和其他7名教师（本杰明·斯沃茨博士、安雅·穆勒博士、布拉德利·法尔曼博士、安东·詹森博士、道格拉斯·斯旺森博士、来自中密歇根大学（CMU）化学和生物化学系的Janice Tomasik和Dale LeCaptain）正在购买配备两个软电离源的电喷雾电离（ESI）-四极杆飞行时间（QTOF）质谱仪（MS（ESI用于易电离的分子，大气压化学电离用于不易电离的分子）、HPLC、二极管阵列检测器和自动进样器。质谱（MS）是一种重要的分析方法，用于通过精确的质量测量来帮助识别和表征复杂样品中的未知化学物质。因此，它的可用性对合成新分子的化学家至关重要。该部门的研究优势在于先进的材料合成，如树枝状分子，碳水化合物，聚合物和电池材料。因此，一个MS，可以提供广泛的分子量信息，并使用软电离技术，允许完整的分子被检测到而不经历碎片，将有助于在该部门的各种大型复杂分子的准确质量测量。收购最先进的ESI-QTOF MS将使CMU化学和生物化学系的研究人员能够有效地进行研究，并在尖端仪器上培训本科生/研究生，这将有助于他们获得成功的技能研究生学习或就业。 ESI-QTOF MS奖旨在加强各级的研究和教育。ESI-QTOF MS提供广泛的质量检测范围，准确的质量，高分辨率/灵敏度和广泛的实验能力，将促进和影响中密歇根大学的各种前沿研究项目。它特别影响涉及化学，生物化学和聚合物/材料科学中新分子合成的领域。获得的ESI-QTOF仪器将用于分析（i）通过化学反应合成的新型强大的生物相关大分子抗氧化剂树枝状分子和树枝状聚合物;（ii）合成的碳水化合物和脂质化学探针，用于研究富含糖脂的分枝杆菌细胞包膜并了解分枝杆菌生理学;（iii）为各种用途而设计的聚合物，例如废水处理、烧伤病人的皮肤支架和燃料电池膜;（iv）为能量储存用途而合成的新电池材料;（v）黄素和烯之间的酶催化反应，以更好地理解它们的作用机制;以及（vi）通过将药物分子缀合到PAMAM树枝状聚合物或树枝状聚合物来合成纳米药物，以克服小药物分子目前存在的问题。具有MS/MS功能的ESI-QTOF MS仪器还将使该部门能够进行蛋白质组学和代谢组学研究，以及确定树枝状聚合物和聚合物材料等大分子的碎片/降解模式。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。

This award is funded by the Major Research Instrumentation Program and the Chemistry Research Instrumentation Program. Professor Choon Young Lee and 7 other faculty (Drs. Benjamin Swarts, Anja Muller, Bradley Fahlman, Anton Jensen, Douglas Swanson, Janice Tomasik and Dale LeCaptain) from the Chemistry and Biochemistry department at Central Michigan University (CMU) are acquiring an Electrospray Ionization (ESI)-Quadrupole Time of Flight (QTOF) Mass Spectrometer (MS) that is equipped with two soft ionization sources (ESI for readily ionizable molecules and atmospheric pressure chemical ionization for not readily ionizable molecules), an HPLC, a Diode Array Detector, and an autosampler. Mass spectrometry (MS) is an essential analytical method used to help identify and characterize unknown chemical species in complex samples through accurate mass measurement. Hence, its availability is vital to chemists who synthesize new molecules. The department’s research strength lies in advanced materials synthesis, such as dendritic molecules, carbohydrates, polymers, and battery materials. Therefore, an MS that can provide a wide range of molecular weight information and that uses soft ionization techniques allowing the intact molecules to be detected without undergoing fragmentation, will facilitate the accurate mass measurements of various large complex molecules made in the department. Acquisition of the state-of-art ESI-QTOF MS will enable researchers in the Department of Chemistry and Biochemistry at CMU to be efficient in their research and train undergraduate/graduate students on a cutting-edge instrument that will help them acquire skills to succeed in post-graduate study or employment. The award of the ESI-QTOF MS is aimed at enhancing research and education at all levels. The ESI-QTOF MS that offers a broad mass detection range, accurate mass, high resolution/sensitivity, and broad experimental capabilities, will facilitate and impact diverse leading-edge research projects at Central Michigan University. It especially impacts areas involving synthesis of new molecules in chemistry, biochemistry and polymer/materials science. The award of an ESI-QTOF instrument will be used in the analyses of (i) novel powerful, biorelevant macromolecular antioxidant dendrons and dendrimers synthesized through chemical reactions; (ii) carbohydrate- and lipid-based chemical probes that are synthesized to study the glycolipid rich mycobacterial cell envelope and understand mycobacterial physiology; (iii) polymers designed for a variety of applications, such as waste-water treatment, skin scaffolds for burn victims, and fuel cell membranes; (iv) new battery materials synthesized for energy storing applications; (v) enzyme-catalyzed reactions between flavins and enes to better understand their mechanisms of action; and (vi) nano-drugs synthesized by conjugating drug molecules to PAMAM dendrimers or encapsulations to overcome the current problems small drug molecules have. The ESI-QTOF MS instrumentation with MS/MS function also will allow the department to perform proteomic and metabolomic studies as well as determine fragmentation/degradation patterns for the large molecules like dendrimers and polymeric materials.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.