eMB: Collaborative Research: ML/AI-assisted environmental scale microbial nonlinear metabolic models

eMB：协作研究：ML/AI 辅助的环境规模微生物非线性代谢模型

• 批准号: 2325171
• 负责人: Tianyu Zhang
• 金额: $ 26.95万
• 依托单位: Montana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2325171&HistoricalAwards=false
• 关键词: eMB; Collaborative; Research; ML; AI

Our world is dominated in many respects by communities of single celled organisms, e.g., bacteria, archaea and non-filamentous fungi. Such communities are key players in all geochemical cycles; they are present in all multicellular organisms, including humans, where in addition to possibly harmful effects, they also have essential beneficial roles. They are ubiquitous in engineered systems as well where, again, they can be beneficial (e.g., waste water treatment) or harmful (e.g., drinking water distribution). In large part, microbial communities interactions with their environments are metabolic through chemicals they take in, products they make with these inputs, and byproducts they excrete, so understanding these metabolic capabilities is essential for understanding how microbes effect their surroundings. Advances in genetic sequencing are making it easier and easier to determine microbial "machinery" (enzymes); researchers are becoming increasingly adept in predicting how this "machinery" combines into "assemblage lines" (metabolic pathways). Armed with this knowledge, the next step is to understand how these "assembly lines" fit into their environment into a sort of large scale "distribution system" that determines overall microbial community function. At large scale, this becomes a challenging computational problem, and current methods are not adequate. This project aims to accelerate these computations by introducing machine learning tools into key bottlenecks in the algorithms. The project is a collaboration between Temple University, Montana State University, and the University of California, San Diego and offers valuable educational, training, and outreach opportunities. Activity funded by this proposal would center on development of computational methods, based on machine learning and artificial intelligence assisted optimization, that are sufficiently efficient so as to make it possible to embed complex cell-scaled models of microbial behavior (metabolic and gene expression models, so-called ME modes) into environmental scale PDE-based models of microbial community activity. In support of this effort, ME models of several specific organisms (S. aureus, S. epidermidis, B. subtilis) will be adapted for use in specific environmental-scale models (basic biofilm communities and built-environment subaerial communities). In complement, environmental-scale continuum-mechanics-based (partial differential equation) models for these systems will be constructed and adapted for use with the new computational methods. AI will also be applied at this macroscale to attempt to identify key metabolic processes at the large scale.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

我们的世界在许多方面都被单细胞生物群落所主宰，例如，细菌、古细菌和非丝状真菌。这类生物群是所有地球化学循环的关键角色;它们存在于包括人类在内的所有多细胞生物体中，除了可能的有害影响外，它们还具有重要的有益作用。它们在工程系统中也是普遍存在的，同样，它们可以是有益的（例如，废水处理）或有害的（例如，饮用水分配）。在很大程度上，微生物群落与环境的相互作用是通过它们摄入的化学物质，它们用这些输入制造的产品以及它们排泄的副产品进行代谢的，因此了解这些代谢能力对于了解微生物如何影响它们的环境至关重要。基因测序的进步使得确定微生物的“机器”（酶）变得越来越容易;研究人员越来越善于预测这种“机器”如何组合成“装配线”（代谢途径）。有了这些知识，下一步就是了解这些“装配线”如何适应它们的环境，成为一种大规模的“分配系统”，决定整体微生物群落的功能。在大规模下，这成为一个具有挑战性的计算问题，目前的方法是不够的。该项目旨在通过将机器学习工具引入算法中的关键瓶颈来加速这些计算。该项目是坦普尔大学、蒙大拿州立大学和加州大学圣地亚哥分校之间的合作，提供了宝贵的教育、培训和推广机会。 由该提案资助的活动将集中在基于机器学习和人工智能辅助优化的计算方法的开发上，这些方法足够有效，以便能够将微生物行为的复杂细胞尺度模型（代谢和基因表达模型，所谓的ME模式）嵌入环境尺度的基于PDE的微生物群落活动模型。为了支持这一努力，ME模型的几个特定的生物体（S。金黄色葡萄球菌、epidermidis，B. B.枯草杆菌）将适用于特定的环境规模模型（基本生物膜群落和建成环境陆上群落）。作为补充，将为这些系统建立环境尺度的基于连续力学的（偏微分方程）模型，并采用新的计算方法。人工智能也将应用于这一宏观尺度，试图在大尺度上识别关键的代谢过程。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。