Collaborative Research: Shedding Light on The Microbial Ecologyand Ecophysiology of Electroactive Anammox Communities
合作研究:揭示电活性厌氧氨氧化群落的微生物生态学和生态生理学
基本信息
- 批准号:2327515
- 负责人:
- 金额:$ 27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2024
- 资助国家:美国
- 起止时间:2024-02-01 至 2027-01-31
- 项目状态:未结题
- 来源:
- 关键词:
项目摘要
Wastewater treatment plants (WWTPs) play a critical role in environmental protection. In the United States, municipal WWTPs process billions of gallons of wastewater every day to remove suspended solids, organic matter, and excess nitrogen nutrients such as ammonium. In most municipal WWTPs, a biological process, activated sludge (AS), is used to remove nitrogen by coupling an aerobic nitrification process which oxidizes ammonium into nitrate followed by an anaerobic denitrification process that reduces the nitrate to benign dinitrogen gas (N2). However, ammonium nitrification in AS reactors requires the supply of oxygen using aeration which requires a significant amount of energy and accounts for 70-80 % of the total energy used in WWTPs. Anaerobic ammonium oxidation (anammox) has emerged as a promising microbial process for removing nitrogen from municipal wastewater with lower energy consumption and operating costs. However, the selection, cultivation, and integration of anammox bacteria into reactors and the treatment trains of municipal WWTPs has remained a challenge due to their slow growth rates and competition from other microorganisms including ammonium oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB). Recent studies show that anammox microbes can generate electricity. Building upon these promising studies, the Principal Investigators (PIs) of this project propose to explore the selection of electroactive anammox bacteria using bioelectrochemical systems with the goal of accelerating their growth, proliferation, and stability in municipal wastewater. The successful completion of this project will benefit society through the generation of fundamental knowledge in environmental microbiology and biotechnology to advance the development and deployment of more cost-effective solutions for nitrogen removal from municipal and industrial wastewater. Additional benefits to society will be achieved through student education and training including the mentoring of one graduate student at Temple University and one graduate student at the University of Maryland.Anaerobic ammonium oxidation (anammox) bacteria have recently been shown to perform extracellular electron transfer (EET), but little is known about the mechanisms through which anammox bacteria transfer electrons extracellularly to electrodes, and even less is known about how electrons are transported intracellularly from the anammoxosome to the outer membrane proteins for downstream EET. The overarching goal of this project is to advance the fundamental understanding of the microbial ecology and ecophysiology of electroactive anammox communities in bioelectrochemical systems (BES). The core and guiding hypothesis of the proposed research is that in BES, EET-dependent anammox is carried out by a microbial population composed of electroactive anammox bacteria and their electroactive partners. Together they form a mutualistic relationship: anammox bacteria fix carbon dioxide and provide organic matter to electroactive bacteria, whose electron shuttles are scavenged by anammox bacteria for EET. If the hypothesis holds, EET-dependent anammox could be electrochemically enhanced through the enrichment of those two partners. The specific aims of the research are to 1) develop electrochemical strategies to build electroactive anammox communities; 2) understand the microbial interactions in electroactive anammox communities; and 3) elucidate the metabolic pathways involved in EET-dependent anammox. The successful completion of this project has the potential for transformative impact through the generation of new fundamental knowledge on the microbial ecology and ecophysiology of electroactive anammox microbiomes in BES to guide the design and implementation of more sustainable technologies and solutions to remove nitrogen from municipal and industrial wastewater. To implement the education and training goals of the project, the PIs propose to leverage existing programs at Temple University (TU) and the University of Maryland (UMD) to recruit and mentor undergraduate students from underrepresented groups to work on the project. In addition, the PIs plan to integrate the findings from this research into existing environmental engineering graduate/undergraduate courses at TU and UMD.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
污水处理厂在环境保护中起着至关重要的作用。在美国,城市污水处理厂每天处理数十亿加仑的废水,以去除悬浮固体、有机物和过量的氮营养物质,如铵。在大多数城市污水处理厂中,生物过程,即活性污泥(AS),通过耦合好氧硝化过程(将铵氧化成硝酸盐)和厌氧反硝化过程(将硝酸盐还原成良性二氮气体(N2))来去除氮。然而,AS反应器中的铵态硝化需要曝气供氧,这需要大量的能量,占污水处理厂总能量的70- 80%。厌氧氨氧化(anammox)是一种很有前途的微生物脱氮工艺,具有较低的能耗和运行成本。然而,厌氧氨氧化菌的选择、培养和整合到反应器和城市污水处理厂的处理序列中仍然是一个挑战,因为它们的生长速度缓慢,并且来自其他微生物,包括氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)的竞争。最近的研究表明厌氧氨氧化微生物可以发电。在这些有前景的研究的基础上,该项目的主要研究者(pi)建议利用生物电化学系统探索电活性厌氧氨氧化菌的选择,目的是加速它们在城市污水中的生长、增殖和稳定性。该项目的成功完成将通过产生环境微生物学和生物技术的基础知识来促进开发和部署更具成本效益的解决方案来去除城市和工业废水中的氮,从而造福社会。通过学生教育和培训,包括指导天普大学的一名研究生和马里兰大学的一名研究生,将为社会带来额外的好处。厌氧氨氧化(anammox)细菌最近被证明可以进行细胞外电子转移(EET),但对厌氧氨氧化细菌将电子在细胞外转移到电极的机制知之甚少,对电子如何在细胞内从厌氧氨氧化体转移到下游EET的外膜蛋白的了解就更少了。本项目的总体目标是促进对生物电化学系统(BES)中电活性厌氧氨氧化群落的微生物生态学和生态生理学的基本认识。本研究的核心和指导假设是,在BES中,依赖eet的厌氧氨氧化是由电活性厌氧氨氧化菌及其电活性伙伴组成的微生物群进行的。它们共同形成了一种互惠关系:厌氧氨氧化菌固定二氧化碳并为电活性细菌提供有机物,电活性细菌的电子梭被厌氧氨氧化菌清除以产生EET。如果这一假设成立,eet依赖性厌氧氨氧化反应可能通过富集这两个伙伴而得到电化学强化。研究的具体目标是:1)制定电化学策略,建立电活性厌氧氨氧化群落;2)了解电活性厌氧氨氧化群落中微生物的相互作用;3)阐明eet依赖性厌氧氨氧化的代谢途径。该项目的成功完成有可能产生变革性的影响,通过在BES中产生关于电活性厌氧氨氧化微生物群的微生物生态学和生态生理学的新基础知识,指导设计和实施更可持续的技术和解决方案,从城市和工业废水中去除氮。为了实现该项目的教育和培训目标,项目负责人建议利用天普大学(TU)和马里兰大学(UMD)的现有项目,从代表性不足的群体中招募和指导本科生参与该项目。此外,pi计划将这项研究的结果整合到TU和UMD现有的环境工程研究生/本科生课程中。该奖项反映了美国国家科学基金会的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(0)
专著数量(0)
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Heyang Yuan其他文献
Enhanced water flux and dewatering using electric-magnetic-responsive hydrogels as draw agents for forward osmosis
使用电磁响应水凝胶作为正向渗透的汲取剂增强水通量和脱水性能
- DOI:
10.1016/j.desal.2025.119040 - 发表时间:
2025-10-15 - 期刊:
- 影响因子:9.800
- 作者:
Mohammadali Vafaei;Sabrina Gerace;Yajing Li;Heyang Yuan;Wen Zhang;Lijie Zhang - 通讯作者:
Lijie Zhang
Inactivation of antibiotic resistant bacteria by ruthenium-doped carbon dots capable of photodynamic generation of intracellular and extracellular reactive oxygen species
通过能够光动力产生细胞内和细胞外活性氧的钌掺杂碳点使抗生素耐药细菌失活
- DOI:
10.1016/j.bioadv.2025.214344 - 发表时间:
2025-11-01 - 期刊:
- 影响因子:6.000
- 作者:
Weibo Xia;Jia Shan;Vladyslav Lutsenko;Zhang Cheng;Yu Liu;Jinjia Xu;Shiqiang Yu;Zheng Peng;Heyang Yuan;Wenfei Hu - 通讯作者:
Wenfei Hu
Bioelectrochemical Systems: Microbiology, Catalysts, Processes and Applications
- DOI:
- 发表时间:
2017-11 - 期刊:
- 影响因子:0
- 作者:
Heyang Yuan - 通讯作者:
Heyang Yuan
Life Cycle Environmental Impact Comparison of Bioelectrochemical Systems for Wastewater Treatment
- DOI:
10.1016/j.procir.2019.01.075 - 发表时间:
2019-01-01 - 期刊:
- 影响因子:
- 作者:
Jingyi Zhang;Heyang Yuan;Ibrahim M Abu-Reesh;Zhen He;Chris Yuan - 通讯作者:
Chris Yuan
Inactivation of antibiotic resistant bacteria by nitrogen-doped carbon quantum dots through spontaneous generation of intracellular and extracellular reactive oxygen species
氮掺杂碳量子点通过自发产生细胞内和细胞外活性氧物种来灭活耐药细菌
- DOI:
10.1016/j.mtbio.2024.101428 - 发表时间:
2025-02-01 - 期刊:
- 影响因子:10.200
- 作者:
Weibo Xia;Zixia Wu;Bingying Hou;Zhang Cheng;Dechuang Bi;Luya Chen;Wei Chen;Heyang Yuan;Leo H. Koole;Lei Qi - 通讯作者:
Lei Qi
Heyang Yuan的其他文献
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{{ truncateString('Heyang Yuan', 18)}}的其他基金
Collaborative Research: Leveraging the interactions between carbon nanomaterials and DNA molecules for mitigating antibiotic resistance
合作研究:利用碳纳米材料和 DNA 分子之间的相互作用来减轻抗生素耐药性
- 批准号:
2307222 - 财政年份:2024
- 资助金额:
$ 27万 - 项目类别:
Standard Grant
EAGER: Chasing the elusive syntrophic partners in direct interspecies electron transfer
EAGER:在直接种间电子转移中追逐难以捉摸的互养伙伴
- 批准号:
2128365 - 财政年份:2021
- 资助金额:
$ 27万 - 项目类别:
Standard Grant
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- 批准号:31224802
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- 批准号:10774081
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