Collaborative Research: RUI: RESEARCH-PGR Meeting Future Food Demands: Phosphoproteomics to Unravel Signaling Pathways in Soybean's Response to Phosphate and Iron Deficiency

合作研究：RUI：RESEARCH-PGR 满足未来食品需求：磷酸蛋白质组学揭示大豆对磷酸盐和铁缺乏的反应的信号通路

• 批准号: 2329894
• 负责人: Robert Chalkley
• 金额: $ 11.52万
• 依托单位: University of California-San Francisco
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-02-01 至 2027-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2329894&HistoricalAwards=false
• 关键词: Collaborative; Research; RUI; RESEARCH; PGR

Soybean is an important crop in the US and worldwide, grown primarily for its oil and as a food source. Soybean crop yield is critical for the US and global economy, but iron and phosphate deficiencies are common problems in many US soybean-growing regions, and this can severely limit soybean production. This problem is exacerbated by the fact that phosphate is a finite resource, and we are at risk of running out of accessible (minable) rock phosphate fertilizer within centuries. The objective of the proposed research is to understand how soybean senses and immediately responds to phosphate and iron deficiencies. Our approach is based on a technique called quantitative phosphoproteomics which has been successfully applied to identify other signaling pathways in plants but has not yet been applied to phosphate or iron deficiency signaling. A better understanding of early responses to two major nutrient stresses could help to develop soybean lines with reduced need for fertilizers. The project will directly impact undergraduate and master students in Science, Technology, Engineering, and Mathematics by providing experience in hands-on research and dissemination. This in turn will help students, mostly belonging to underrepresented minority groups, to obtain employment in the local biotech industry. Avoiding a food crisis is one of the greatest challenges facing the world today, requiring crops with improved uptake and utilization of nutrients. Phosphate and iron deficiencies limit soybean production in the US and worldwide. Although significant research has been devoted to identifying plant responses to phosphate and iron deficiency, far less is known about how plants sense and signal these deficiencies. This is because most experimental approaches have focused on changes in gene expression. However, signal transduction components, while usually not differentially expressed, are frequently differentially phosphorylated. We will apply quantitative phosphoproteomics to soybeans to identify differentially phosphorylated proteins in response to short-term phosphate and iron deficiency. We will then confirm potential signaling roles by generating RNAi knockdown mutants and analyzing these for impaired nutrient signaling responses. While quantitative phosphoproteomics has been successfully applied to unravel other signal transduction pathways in plant stress responses, it has not yet been applied to phosphate or iron deficiency signaling. The proposed research will help bridge the gap between known loci and genes involved in nutrient tolerance in soybeans and the underlying signaling pathways. In the long term, a better understanding of signal transduction and networks integrating various nutrient stress responses could aid system-based approaches to breed or bioengineer soybeans with increased stress tolerance, productivity, and reduced need for fertilizers. Our educational objective is to train undergraduate and graduate students, most belonging to underrepresented minority groups in STEM, in hands-on research and dissemination.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

大豆是美国和全世界的重要作物，主要种植其油和作为食物来源。大豆作物产量对美国和全球经济至关重要，但铁和磷缺乏是美国许多大豆种植区的常见问题，这可能严重限制大豆产量。磷酸盐是一种有限的资源，这一事实加剧了这个问题，我们面临着在几个世纪内用完可获得（可开采）的磷矿肥料的风险。这项研究的目的是了解大豆如何感知并立即对磷酸盐和铁缺乏做出反应。我们的方法是基于一种称为定量磷酸蛋白质组学的技术，该技术已成功应用于识别植物中的其他信号通路，但尚未应用于磷酸盐或缺铁信号。更好地了解两种主要营养胁迫的早期反应有助于开发减少肥料需求的大豆品系。该项目将通过提供实践研究和传播经验，直接影响科学，技术，工程和数学的本科生和硕士生。这反过来又将有助于学生，其中大多数属于代表性不足的少数群体，在当地生物技术行业就业。避免粮食危机是当今世界面临的最大挑战之一，需要作物对营养的吸收和利用得到改善。磷和铁的缺乏限制了美国和世界范围内的大豆生产。虽然大量的研究致力于确定植物对磷酸盐和铁缺乏的反应，但对植物如何感知和发出这些缺乏信号的了解却少得多。这是因为大多数实验方法都集中在基因表达的变化上。然而，信号转导成分，虽然通常不差异表达，但经常差异磷酸化。我们将应用定量磷酸蛋白质组学对大豆，以确定差异磷酸化蛋白质在短期磷酸盐和铁缺乏的反应。然后，我们将通过产生RNAi敲低突变体并分析这些受损的营养信号传导反应来确认潜在的信号传导作用。虽然定量磷酸蛋白质组学已成功地应用于解开其他信号转导途径在植物胁迫反应，它还没有被应用到磷酸盐或缺铁信号。这项拟议中的研究将有助于弥合大豆中与营养耐受性有关的已知位点和基因与潜在信号通路之间的差距。从长远来看，更好地了解信号转导和整合各种营养胁迫反应的网络可以帮助基于系统的方法来培育或生物工程大豆，提高胁迫耐受性，生产力和减少对肥料的需求。我们的教育目标是培养本科生和研究生，其中大部分属于STEM领域代表性不足的少数群体，进行实践研究和传播。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响力审查标准进行评估，被认为值得支持。