Spatial and temporal coordination of RNA translation, replication and assembly in a membranous plus-strand RNA virus replication factory (11)

我们在确定正链RNA病毒复制细胞器(RO)的生物发生和功能方面取得了重要进展，并揭示了丙型肝炎病毒和SARS CoV 2之间惊人的相似之处。此外，我们通过采用单细胞分辨率的集成数学模型，确定了登革热复制中的一个敏感步骤，证明该步骤非常适合抗病毒治疗。在下一个资助期，我们希望继续进行病毒和宿主细胞因子的比较分析，这些因素涉及RO的生物发生和系统发育上遥远的病毒的功能。将包括生物信息学和系统生物学方法，以捕捉RO形成、病毒复制和病毒粒子组装的动态。

We made important progress in identifying relevant viral and cellular factors in plus-strand RNA virus replica-tion organelle (RO) biogenesis and functionality, and revealed surprising parallels between HCV and SARS CoV 2. Furthermore, we identified a sensitive step in Dengue replication that turned out to be well suited for antiviral therapy by employing an integrated mathematical model at single-cell resolution. In the next funding period, we want to continue conducting comparative analyses of viral and host cell factors involved in RO biogenesis and function across phylogenetically distant viruses. Bioinformatics and systems biology approaches will be included to capture the dynamics of RO formation, viral replication and virion assembly.