Visualising reovirus infection in polarised epithelium: From touchdown to antiviral innate immune response (14)

极化上皮中呼肠孤病毒感染的可视化：从着陆到抗病毒先天免疫反应 (14)

• 批准号: 258370718
• 金额: --
• 依托单位: Heidelberger Akademie der Wissenschaften
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/258370718?language=en
• 关键词: Visualising; reovirus; infection; polarised; epithelium

Intestinal epithelial cells (IECs) constitute the primary barrier for enteric pathogens. This project aims at achieving a comprehensive characterization of host/pathogen interactions by integrating, at the single cell level, the intrinsic innate immune response by IECs within the physiological crypt/villi axis organization of the intestinal epithelium. In collaboration with Höfer and Lusic, we will perform a comprehensive characterization of the functional differ-ences of type I and III IFNs in primary IECs. Together with Dao Thi and Kräusslich, using human organoids, we will address how IECs mediate an asymmetric immune response and characterize the importance of autocrine and/or paracrine signals in the protection of cells during pathogen spread within a tissue-like environment.

肠上皮细胞(IECS)是肠道病原体的主要屏障。该项目旨在通过在单细胞水平上整合IECS在肠上皮生理隐窝/绒毛轴组织内的固有固有免疫反应来实现宿主/病原体相互作用的全面表征。我们将与Höfer和Lusic合作，对初级IEC中I型和III型IFN的功能差异进行全面的表征。我们将与Dao Thi和Kräusslich一起，使用人类有机体，研究IECS如何调节不对称免疫反应，并表征自分泌和/或旁分泌信号在病原体在类似组织的环境中传播时保护细胞的重要性。