Doctoral Dissertation Research: Investigating the genomic underpinnings of the human hand and foot

博士论文研究：研究人类手脚的基因组基础

• 批准号: 2337516
• 负责人: Terence Capellini
• 金额: $ 4.23万
• 依托单位: Harvard University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2024
• 资助国家: 美国
• 起止时间: 2024-02-15 至 2026-01-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2337516&HistoricalAwards=false
• 关键词: Doctoral; Dissertation; Research; Investigating; genomic

Humans use their feet almost exclusively in locomotion (walking, running, etc.) while our hands are used for a range of activities involving precise, powerful movements of individual fingers, such as typing on a computer, playing the piano, or throwing a baseball. The ability to perform these tasks requires that the bones of the hand and foot have an appropriate size and shape. Human hand and foot bones have some key differences in structure from our closest living primate relative and these differences are encoded in the genome by DNA sequences which differ between the two species. In this doctoral dissertation project, thousands of human and non-human primate DNA sequences are screened to identify underlying genomic regions that are likely to encode the differences observed. The project advances knowledge about the developmental genetics of the hand and foot in the context of human evolution and supports undergraduate and graduate training, including students from groups underrepresented in STEM.Numerous studies have identified thousands of genomic regions with an excess of human-specific DNA changes. Many of these regions were likely under positive selection over the course of human evolution, but tying specific genetic changes to phenotypic change remains a challenge akin to finding a needle in a haystack. To identify genetic changes underlying uniquely human features of the hand and foot skeleton, this proposal uses the massively parallel reporter assay (MPRA) system to simultaneously screen the human and chimp versions of thousands of genomic regions known to be involved in the developing human hand or foot for differential activity between the two species in cartilage cells. This research can shed light on the genetic architecture patterning individual elements of the hand and foot skeleton, which can inform our understanding of human evolution, development, and disease.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

人类几乎只在运动中使用脚（行走，跑步等）。而我们的手用于一系列活动，包括精确的，有力的单个手指的运动，如在电脑上打字，弹钢琴，或扔棒球。执行这些任务的能力需要手和脚的骨头具有适当的大小和形状。人类的手骨和脚骨在结构上与我们最近的灵长类亲属有一些关键的差异，这些差异在基因组中由两个物种之间不同的DNA序列编码。在这个博士论文项目中，筛选了数千个人类和非人类灵长类动物的DNA序列，以确定可能编码观察到的差异的潜在基因组区域。该项目在人类进化的背景下推进了关于手和脚的发育遗传学的知识，并支持本科生和研究生的培训，包括来自STEM中代表性不足的群体的学生。许多研究已经确定了数千个具有过量人类特异性DNA变化的基因组区域。在人类进化过程中，这些区域中的许多区域可能处于正选择之下，但将特定的遗传变化与表型变化联系起来仍然是一个类似于大海捞针的挑战。为了识别手和脚骨骼独特的人类特征的遗传变化，该提案使用大规模并行报告分析（MPRA）系统同时筛选人类和黑猩猩版本的数千个已知参与人类手或脚发育的基因组区域，以确定两个物种之间软骨细胞的差异活性。这项研究可以揭示手和脚骨骼的遗传结构模式的个别元素，这可以告知我们对人类进化，发展和疾病的理解。这个奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。