Regulatory molecular networks underpinning periderm development

支持周皮发育的调控分子网络

• 批准号: 259156069
• 负责人: Professorin Dr. Laura Ragni, Ph.D.
• 金额: --
• 依托单位: Arbeitsgruppe Zellbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/259156069?language=en
• 关键词: Regulatory; molecular; networks; underpinning; periderm

The periderm, as an armor, protects the plant vasculature from biotic and abiotic stresses. In many seed plants, it is formed from the meristematic cells of the cork cambium during the increase of girth of plant organs. Similarly, to the vascular cambium, the cork cambium is arranged as a cylinder of cells that divide bifacially and differentiate into cork cells toward the environment and into phelloderm cells toward the vasculature. We previously showed that the plant hormone auxin is required for the initiation and maintenance of the cork cambium in roots and that the transcription factors WOX4 and BP act downstream of auxin. Strikingly, auxin is also required for vascular cambium establishment and WOX4 and BP are known vascular cambium regulators, raising questions on how auxin specificity is achieved and whether stem cell identity differs among cambia. By employing genetic tools, which specifically hinder auxin signaling in the cork or vascular cambium in a transcriptomic experiment, we have identified new putative cork cambium specific and core cambial regulators. In the continuation of this project, exploiting resources in which the expression of these novel regulators can be augmented or reduced in a specific stem cell context, we aim to decipher the cork and vascular cambium regulatory networks and gain more insights into the integration/communication of the two cambia. At the same time, we address the molecular determinants of cork cambium identity by employing single cell transcriptome approaches. Finally, we seek to understand how the environment modulates cork and vascular cambium activities, which will pave the way to identify novel root traits for breeding programs aiming to improve plant resilience.

周皮作为一种盔甲，保护植物的维管系统免受生物和非生物胁迫。在许多种子植物中，它是在植物器官周长增加的过程中由软木形成层的分生组织细胞形成的。与维管形成层类似，木栓形成层排列为一个细胞圆柱体，两面分裂，向环境分化为木栓细胞，向维管系统分化为木栓层细胞。我们之前的研究表明，植物激素生长素是根中软木形成层的启动和维持所必需的，转录因子WOX4和BP作用于生长素的下游。值得注意的是，生长素也是维管形成层建立所必需的，而WOX4和BP是已知的维管形成层调节因子，这就提出了生长素专一性是如何实现的以及不同形成层之间干细胞特性是否不同的问题。通过利用基因工具，在转录转录实验中特异性地阻碍软木或维管形成层中的生长素信号，我们已经确定了新的假定的软木形成层特定的和核心的形成层调节因子。在这个项目的继续中，利用在特定干细胞背景下这些新型调节子的表达可以增强或减少的资源，我们的目标是破译木栓和维管形成层调节网络，并获得更多关于这两个形成层的整合/交流的见解。同时，我们利用单细胞转录组的方法研究了软木形成层特性的分子决定因素。最后，我们试图了解环境如何调节软木和维管形成层的活动，这将为识别新的根性状为旨在提高植物抗逆性的育种计划铺平道路。