Molecular mechanisms underlying cork barrier differentiation: the role of MYBs transcription factors

软木屏障分化的分子机制：MYB转录因子的作用

• 批准号: 453092819
• 负责人: Professorin Dr. Laura Ragni, Ph.D.
• 金额: --
• 依托单位: Arbeitsgruppe Zellbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/453092819?language=en
• 关键词: Molecular; mechanisms; underlying; cork; barrier

Like our skin, the outermost tissues of the plant body, evolved to protect the plant against biotic and abiotic agents and to control loss of water and gas exchanges. The periderm, is the outer tissue that covers and protects the vasculature during secondary growth. It originates from the cork cambium, which divides and produces inward the phelloderm and outward the cork. The barrier properties of the periderm are conferred by the cork, which accumulates high amount of suberin and lignin in the cell walls. Despite the economic and agronomical importance of the cork, few regulators of periderm formation and cork differentiation are known. Based on periderm/cork transcript-profiling of several species, we identified a set of closely related MYB transcription factors (TFs), which are conserved among species and specifically expressed in the periderm/cork. Our preliminary results, exploiting the Arabidopsis root, as a model, suggest a dual function in cork differentiation for these MYBs. On one hand, they promote suberin accumulation and on the other hand they repress cork cambium activity. Here, exploiting tools and resources in which TFs expression in the periderm can be induced in a precisely defined spatiotemporal context, we aim to further dissect their role during cork differentiation, to identify their targets and thus establish the first cork regulatory network. In addition, we will focus on their function during abiotic stresses, paving the way for breeding programs targeting root barriers.

像我们的皮肤一样，植物体的最外面组织也进化为保护植物免受生物和非生物剂的侵害，并控制水和气体交换的损失。 Periderm是覆盖并保护二次生长期间脉管系统的外部组织。它起源于软木形成，该软木cambium分裂并向内生产佩皮尔和向外的软木塞。 periderm的屏障特性由软木塞赋予，该特性在细胞壁中积累了大量的suberin和木质素。尽管软木塞具有经济和农艺的重要性，但佩里德尔形成和软木差分的调节剂很少。基于几种物种的Periderm/Cork转录本，我们确定了一组密切相关的MYB转录因子（TFS），这些因子在物种之间保守，并在Periderm/Cork中特别表达。我们的初步结果利用了拟南芥根作为模型，这表明这些MYB的软木分化具有双重功能。一方面，它们促进了suberin的积累，另一方面，它们压制了软木形成性活动。在这里，利用TFS在Periderm中表达的工具和资源可以在精确定义的时空环境中诱导，我们旨在进一步剖析其在软木区分化过程中的作用，以识别其目标，从而建立第一个软木监管网络。此外，我们将重点关注它们在非生物压力期间的功能，为针对根屏障的育种计划铺平道路。