Molecular mechanisms underlying cork barrier differentiation: the role of MYBs transcription factors

软木屏障分化的分子机制：MYB转录因子的作用

• 批准号: 453092819
• 负责人: Professorin Dr. Laura Ragni, Ph.D.
• 金额: --
• 依托单位: Arbeitsgruppe Zellbiologie der Pflanzen
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/453092819?language=en
• 关键词: Molecular; mechanisms; underlying; cork; barrier

Like our skin, the outermost tissues of the plant body, evolved to protect the plant against biotic and abiotic agents and to control loss of water and gas exchanges. The periderm, is the outer tissue that covers and protects the vasculature during secondary growth. It originates from the cork cambium, which divides and produces inward the phelloderm and outward the cork. The barrier properties of the periderm are conferred by the cork, which accumulates high amount of suberin and lignin in the cell walls. Despite the economic and agronomical importance of the cork, few regulators of periderm formation and cork differentiation are known. Based on periderm/cork transcript-profiling of several species, we identified a set of closely related MYB transcription factors (TFs), which are conserved among species and specifically expressed in the periderm/cork. Our preliminary results, exploiting the Arabidopsis root, as a model, suggest a dual function in cork differentiation for these MYBs. On one hand, they promote suberin accumulation and on the other hand they repress cork cambium activity. Here, exploiting tools and resources in which TFs expression in the periderm can be induced in a precisely defined spatiotemporal context, we aim to further dissect their role during cork differentiation, to identify their targets and thus establish the first cork regulatory network. In addition, we will focus on their function during abiotic stresses, paving the way for breeding programs targeting root barriers.

就像我们的皮肤一样，植物体的最外层组织进化成保护植物免受生物和非生物因子的侵害，并控制水和气体交换的损失。围壁是在次生生长期间覆盖和保护脉管系统的外部组织。它起源于软木形成层，软木形成层向内分裂并产生外胚层，向外分裂并产生软木。围壁的屏障特性由软木赋予，软木在细胞壁中积累大量的木栓质和木质素。尽管软木的经济和农艺学的重要性，很少的调节器的围壁形成和软木分化是已知的。基于几个物种的围隔/软木转录谱，我们确定了一组密切相关的MYB转录因子（TF），这是物种之间的保守和特异性表达的围隔/软木。我们的初步研究结果，利用拟南芥根，作为一个模型，表明软木分化的双重功能，这些MYBs。它们一方面促进木栓质的积累，另一方面抑制木栓形成层的活动。在这里，利用工具和资源，其中TF的表达在围产期可以诱导在一个精确定义的时空背景下，我们的目标是进一步剖析他们在软木分化的作用，以确定他们的目标，从而建立第一个软木调控网络。此外，我们将重点关注它们在非生物胁迫下的功能，为针对根障碍的育种计划铺平道路。