Redistribution of water via mycelia networks of saprotrophic fungi and its effect on C-mineralization in desiccated soils

通过腐生真菌菌丝体网络进行水的重新分配及其对干燥土壤中碳矿化的影响

• 批准号: 260518461
• 负责人: Professor Dr. Egbert Matzner
• 金额: --
• 依托单位: Lehrstuhl für Bodenökologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/260518461?language=en
• 关键词: Redistribution; water; via; mycelia; networks

Desiccation of the upper soil during the growing season is common in temperate climate. Soil desiccation is known to reduce C mineralization substantially. However, redistribution of water by mycelia of saprotrohic fungi (hydraulic redistribution) from moist to dry zones of the soil might mitigate the effects of desiccation of C mineralization, providing a larger competitiveness of saprotrophic fungi under dry conditions. Here, we will investigate if mycelia networks of saprotrophic fungi contribute to water redistribution in soil and how this influences mineralization of organic matter. Mesocosms will be used comprising two chambers, separated by a 2 mm air gap to prevent bulk flow of water and allowing only water flow via fungal mycelia. After the mycelia connection between both chambers is established, both chambers will be desiccated to a soil water tension of pF 4 - 5. Subsequently, one chamber will be reirrigated with deuterium labelled water and a florescent marker. At the end of the experiment, soil samples as well as hyphae of the desiccated chamber will be analysed for stable isotope content and fluorescent labelling. In a second set of experiments double 15N/13C labelled plant material will be exposed in the non-irrigated desiccated chamber. The effect of hydraulic redistribution on the mineralization will be studied by measuring CO2 evolution and the 13CO2 signal. 13C and 15N isotopes will be analyzed in soil samples of the irrigated chambers to determine the percentage of retransferred N and C. Furthermore, the impact of hydraulic redistribution on fungal enzyme activity will be analyzed at high spatial resolution using soil zymography. These experiments will shed new light on the role of fungal mycelia for mineralization under dry soil conditions.

在温带气候中，上层土壤在生长季节干燥是很常见的。已知土壤干燥会显著减少碳矿化。然而，腐解真菌菌丝体的水分再分配（水力再分配）从潮湿到干燥区的土壤可能会减轻干燥的C矿化的影响，提供了更大的竞争力，腐解真菌在干燥条件下。在这里，我们将调查，如果腐食真菌的菌丝体网络有助于土壤中的水分再分配，以及这如何影响有机质的矿化。将使用包括两个室的中围，两个室由2 mm的气隙隔开以防止水的大量流动，并且仅允许水通过真菌菌丝体流动。在两个室之间建立菌丝体连接之后，将两个室干燥至pF 4 - 5的土壤水张力。随后，用氘标记水和荧光标记物对一个腔室进行再灌注。在实验结束时，将分析土壤样品以及干燥室中的菌丝的稳定同位素含量和荧光标记。在第二组实验中，双15 N/13 C标记的植物材料将暴露在非灌溉干燥室中。将通过测量CO2演化和13 CO2信号来研究水力再分配对矿化的影响。13 C和15 N同位素将在灌溉室的土壤样品中进行分析，以确定再转移的N和C的百分比。此外，水力再分配对真菌酶活性的影响将在高空间分辨率下使用土壤酶谱分析。这些实验将为真菌菌丝体在干燥土壤条件下的矿化作用提供新的线索。

项目成果

Vitamin B2 (riboflavin) increases drought tolerance of Agaricus bisporus

• DOI: 10.1080/00275514.2017.1414544
• 发表时间: 2017-01-01
• 期刊: MYCOLOGIA
• 影响因子: 2.8
• 作者: Guhr, Alexander;Horn, Marcus A.;Weig, Alfons R.
• 通讯作者: Weig, Alfons R.

Redistribution of soil water by a saprotrophic fungus enhances carbon mineralization

• DOI: 10.1073/pnas.1514435112
• 发表时间: 2015-11-24
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Guhr, Alexander;Borken, Werner;Matzner, Egbert
• 通讯作者: Matzner, Egbert

Effect of water redistribution by two distinct saprotrophic fungi on carbon mineralization and nitrogen translocation in dry soil

• DOI: 10.1016/j.soilbio.2016.09.009
• 发表时间: 2016-12-01
• 期刊: SOIL BIOLOGY & BIOCHEMISTRY
• 影响因子: 9.7
• 作者: Guhr, Alexander;Marzini, Carlo;Matzner, Egbert
• 通讯作者: Matzner, Egbert