Association mapping of net blotch and spot blotch resistance in a set of Hordeum vulgare accessions originated from the centers of barley diversity

一组源自大麦多样性中心的大麦种质中网斑病和斑斑病抗性的关联图谱

• 批准号: 261296357
• 负责人: Professor Dr. Frank Ordon
• 金额: --
• 依托单位: Institut für Resistenzforschung und Stresstoleranz
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/261296357?language=en
• 关键词: Association; mapping; net; blotch; spot

Net blotch caused by Pyrenophora teres and spot blotch caused by Cochliobolus sativus are two most damaging and widely distributed fungal pathogens of barley. As a result of long-term joint research projects of the Laboratory of Plant Resistance to Diseases of the All-Russian Research Institute of Plant Protection (VIZR) and the Institute for Resistance Research and Stress Tolerance of the Federal Research Center for Cultivated Plants (JKI), 10.000 barley accessions from different genetic centers of barley diversity and commercial cultivars were evaluated for resistance to both P. teres and C. sativus, and as a result about 450 accessions with different levels of resistance were identified. However, up to now very limited or no information on major genes or QTL involved in resistance of these accessions is available. To get this information in such a large number of accessions, genome wide association studies are an efficient tool facilitating the analyses of a much broader diversity than analyses in bi-parental populations.The objectives of the project are (i) to determine the genetic diversity of resistance and identify QTL for resistance to P. teres f. teres, and P. teres f. maculata as well as to C. sativus in a representative barley germplasm collection employing genome wide association studies (GWAS), (ii) to test the stability and transferability of some detected QTL by integrating associated markers in segregating already phenotyped DH-populations, (iii) to saturate these QTL with markers and to link these to the physical and sequence maps of barley towards the identification of candidate genes, (iv) to enhance the use of these QTL in breeding by developing efficient and cheap PCR assays. To achieve this, 450 barley accessions will be phenotyped at the JKI and the VIZR for resistance by inoculation with different isolates of P. teres f. teres, P. teres f. maculata, and C. sativus originated from Russia and Germany to get detailed information on resistance. For genotyping, the Illumina 9k iSelect barley Chip and genotyping by sequencing (GBS) currently under establishment at JKI will be used and population structure will be estimated on two SSRs per chromosome arm. GWAS will be conducted using a mixed linear model approach. Based on these GWAS it is expected that most of the genomic regions of barley encoding resistance to P. teres f. teres, P. teres f. maculata, and C. sativus will be identified. Respective QTL regions will be marker saturated as a first step towards a map based cloning approach. Using this approach detailed information on the genetic diversity of resistance concerning the above mentioned pathogens will be gained and respective markers will facilitate a directed use of this diversity in barley breeding.

由嗜血杆菌和由小肠sativus引起的斑岩菌和斑点斑点引起的净斑点是大麦的两个最具破坏性和广泛分布的真菌病原体。 As a result of long-term joint research projects of the Laboratory of Plant Resistance to Diseases of the All-Russian Research Institute of Plant Protection (VIZR) and the Institute for Resistance Research and Stress Tolerance of the Federal Research Center for Cultivated Plants (JKI), 10.000 barley accessions from different genetic centers of barley diversity and commercial cultivars were evaluated for resistance to both P. teres and C. sativus, and as a result about 450 accessions with different levels of鉴定了电阻。但是，直到现在非常有限或没有有关主要基因或QTL涉及这些加入抗性的信息。为了在如此大量的加入中获取这些信息，基因组广泛的关联研究是一种有效的工具，该工具促进了与双期育种人群中分析更广泛多样性的分析。该项目的目标是（i）确定电阻的遗传多样性并识别QTL以识别QTL的抗性。 Teres和P. teres f。在代表性的大麦种质收集中，使用基因组广泛的关联研究（GWAS），（ii）测试某些检测到的QTL的稳定性和可传递性，通过将相关的标记整合在隔离已经表现为DH的POPOPUTION，（iii）与这些QTL饱和到物理（III）符合物理（III）的物理（III），以将物理（III）与物理（cand）相连（III），以链接到物理（III）的物理（III），以链接到物理（III）的物理（III），以链接到物理（III）的物理（III），以将物理（III）与物理（cand iiv）相连（III通过开发高效且廉价的PCR分析，可以增强这些QTL在繁殖中的使用。为此，将在JKI和VIZR上表型450个大麦饰品，以通过使用不同分离株的P. teres f接种来抗性。 Teres，P。Teres f。 Maculata和C. sativus起源于俄罗斯和德国，以获取有关抵抗的详细信息。对于基因分型，Illumina 9K Iselect大麦芯片和通过测序（GB）（GB）目前正在JKI建立的基因分型（GBS），并且将估算每个染色体组的两个SSR估计的种群结构。 GWA将使用混合线性模型方法进行。基于这些GWA，可以预期大麦的大多数基因组区域编码对P. teres f的抗性。 Teres，P。Teres f。将确定MacUlata和C. sativus。各自的QTL区域将被标记为迈向基于地图的克隆方法的第一步。使用这种方法，将获得有关上述病原体抗药性的遗传多样性的详细信息，并且各自的标记将有助于将这种多样性的定向使用在大麦育种中。