Dynamic aspects of memory formation. - Deconstructing principles on the molecular, neuronal and network level.

记忆巩固的概念不受连续的生理过程的支持，在细胞和分子水平上也没有简单的补充。相反，记忆能力似乎依赖于一个由不同的分子和神经元过程组成的复杂网络，这些过程在不同的时间点不连续地支持可塑性。普遍的发现是，特定记忆成分的形成需要特定的时间模式的训练。在这里，我建议解构设计原则，这些原则允许特定的行为事件年表从许多可能的机制中只选择一个来影响特定类型突触的突触效率以支持记忆。我将利用果蝇厌恶气味的学习来回答这个问题，强调三个层次的复杂性。在神经元网络的层面上，我的目标是了解记忆形成过程中神经元回路的动态组织。在分子水平上，我的目标是研究Dunce磷酸二酯酶亚型对记忆形成动态的影响。在生理水平上，我将重点研究突触圈套复合体的负调节因子--Tomosyn的动态磷酸化。综上所述，这些目标将揭示支持记忆的通用cAMP信号通路的功能链的设计。

The concept of memory consolidation is not supported by a continuous physiological process and has no simple complement at the cellular and molecular level. Rather it appears that memory performance relies on a complex network of distinct molecular and neuronal processes that discontinuously support plasticity at different time points. It is a universal finding that formation of a particular memory component requires a particular temporal pattern of training. Here, I propose to deconstruct the design principles that allow a particular chronology of behavioral events to select just one out of many possible mechanism to impact on synaptic efficacy of a particular type of synapses to support memory. I will engage this question by use of Drosophila aversive odor learning emphasizing three levels of complexity. At level of neuronal networks I aim to understand the dynamic organization of neuronal circuits during the process of memory formation. At the molecular level I aim on the impact of dunce phosphodiesterase isoforms on the dynamic of memory formation. At the physiological level I will focus on dynamic phosphorylation of Tomosyn, a negative regulator of synaptic SNARE complexes. Taken together, these aims will reveal the design of a functional chain along the universal cAMP signaling pathway in support of memory.