Dynamic aspects of memory formation. - Deconstructing principles on the molecular, neuronal and network level.

记忆巩固的概念没有连续的生理过程支持，在细胞和分子水平上也没有简单的补充。相反，记忆表现似乎依赖于一个复杂的网络，该网络由不同的分子和神经元过程组成，在不同的时间点不连续地支持可塑性。这是一个普遍的发现，形成一个特定的记忆成分需要一个特定的时间模式的培训。在这里，我建议解构的设计原则，允许一个特定的行为事件的年表，选择只有一个从许多可能的机制，以影响突触的特定类型的突触，以支持记忆的功效。我将通过使用果蝇厌恶气味学习强调三个层次的复杂性来处理这个问题。在神经元网络层面，我的目标是了解记忆形成过程中神经元回路的动态组织。在分子水平上，我的目标是研究笨蛋磷酸二酯酶同工酶对记忆形成动力学的影响。在生理水平上，我将集中在Tomosyn的动态磷酸化，突触陷阱复合物的负调节。综合起来，这些目标将揭示一个功能链的设计沿着支持记忆的通用cAMP信号通路。

The concept of memory consolidation is not supported by a continuous physiological process and has no simple complement at the cellular and molecular level. Rather it appears that memory performance relies on a complex network of distinct molecular and neuronal processes that discontinuously support plasticity at different time points. It is a universal finding that formation of a particular memory component requires a particular temporal pattern of training. Here, I propose to deconstruct the design principles that allow a particular chronology of behavioral events to select just one out of many possible mechanism to impact on synaptic efficacy of a particular type of synapses to support memory. I will engage this question by use of Drosophila aversive odor learning emphasizing three levels of complexity. At level of neuronal networks I aim to understand the dynamic organization of neuronal circuits during the process of memory formation. At the molecular level I aim on the impact of dunce phosphodiesterase isoforms on the dynamic of memory formation. At the physiological level I will focus on dynamic phosphorylation of Tomosyn, a negative regulator of synaptic SNARE complexes. Taken together, these aims will reveal the design of a functional chain along the universal cAMP signaling pathway in support of memory.