Impact of Light on Differentiation and Virulence of Botrytis cinerea

光对灰葡萄孢分化和毒力的影响

基本信息

  • 批准号:
    266542482
  • 负责人:
  • 金额:
    --
  • 依托单位:
  • 依托单位国家:
    德国
  • 项目类别:
    Research Grants
  • 财政年份:
    2014
  • 资助国家:
    德国
  • 起止时间:
    2013-12-31 至 2017-12-31
  • 项目状态:
    已结题

项目摘要

The ascomycetous fungus Botrytis cinerea has a worldwide distribution and is the causal agent of gray mold diseases in more than 235 plants species including high-value crops such as grape vine and strawberry. The fungus may reproduce asexually by forming macroconidia for dispersal (summer cycle) and sclerotia for survival (winter cycle); the latter also participate in sexual reproduction by bearing the apothecia after fertilization by microconidia has taken place. Light induces the differentiation of conidia and apothecia, while sclerotia are exclusively formed in the absence of light. Field populations are highly diverse with regard to different phenotypic traits and can be divided into two groups: strains that undergo photomorphogenesis (LIGHT-RESPONSIVE) and BLIND strains that produce either sterile mycelia (FLUFFY) or the same reproductive structures under different light conditions (ALWAYS CONIDIA, ALWAYS SCLEROTIA). However, the inability to form certain reproductive structures such as conidia as the major source of inoculum or sclerotia as prerequisite for sexual recombination is expected to decrease the overall fitness of the pathogen, and thus, it is questionable why BLIND strains are abundantly found in the field. In fact, the BLIND phenotype may accompanied by reduced virulence, as shown for the ALWAYS CONIDIA phenotype of strains T4 and 1750 that is caused by single nucleotide polymorphisms (SNPs) in the gene bcvel1. The identification of bcltf1 (Light-responsive Transcription Factor 1) as new virulence-associated gene by random mutagenesis, further supports our hypothesis of an interrelationship between light signaling and virulence. Accordingly, we assume that the same mechanisms (e.g. expression of detoxifying enzymes, formation of pigments) are activated and required for light tolerance and virulence by protecting the fungus from oxidative stress arising during illumination (photooxidative stress) and host infection (oxidative burst). In view of the impact of light on the life cycle of B. cinerea and the limited knowledge about light signaling in plant pathogenic fungi in general, we aim to investigate the network of photoreceptors and light-responsive transcription factors that regulates the differentiation programs in response to the light, pigment formation and virulence. In addition, a FORWARD GENETICS approach is intended to identify further components of the light signaling machinery in B. cinerea.
子囊菌属真菌灰葡萄孢(Botrytis cinerea)具有世界性分布,并且是超过235种植物物种(包括高价值作物如葡萄藤和草莓)中的灰霉病的病原体。该真菌可以通过形成大分生孢子进行无性繁殖(夏季循环)和形成菌核进行生存(冬季循环);后者也参与有性繁殖,在小分生孢子受精后产生孢子囊。光诱导分生孢子和子囊盘的分化,而菌核仅在没有光的情况下形成。田间种群在不同的表型性状方面具有高度多样性,可以分为两组:经历光形态建成的菌株(光响应型)和在不同光照条件下产生无菌菌丝体(蓬松型)或相同生殖结构(始终分生孢子,始终硬化型)的盲菌株。然而,不能形成某些生殖结构,例如作为接种物的主要来源的分生孢子或作为有性重组的先决条件的菌核,预计会降低病原体的整体适合度,因此,在田间大量发现BLIND菌株的原因值得怀疑。事实上,BLIND表型可能伴随毒力降低,如菌株T4和1750的ALWAYS CONIDIA表型所示,其由基因bcvel 1中的单核苷酸多态性(SNP)引起。通过随机突变鉴定出新的毒力相关基因bcltf 1(Light-responsive Transcription Factor 1),进一步支持了我们关于光信号与毒力相关的假说。因此,我们假设相同的机制(例如解毒酶的表达,色素的形成)被激活,并通过保护真菌免受光照(光氧化应激)和宿主感染(氧化爆发)过程中产生的氧化应激而获得光耐受性和毒力。鉴于光照对B生命周期的影响。灰霉病和有限的知识,在植物病原真菌的光信号一般情况下,我们的目标是研究光受体和光响应转录因子的网络,调节分化程序响应于光,色素形成和毒力。此外,正向遗传学方法旨在鉴定B中光信号机制的其他组分。灰叶

项目成果

期刊论文数量(7)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The Two Cryptochrome/Photolyase Family Proteins Fulfill Distinct Roles in DNA Photorepair and Regulation of Conidiation in the Gray Mold Fungus Botrytis cinerea
The putative H3K36 demethylase BcKDM1 affects virulence, stress responses and photomorphogenesis in Botrytis cinerea.
  • DOI:
    10.1016/j.fgb.2018.11.003
  • 发表时间:
    2019-02
  • 期刊:
  • 影响因子:
    0
  • 作者:
    J. Schumacher;L. Studt;P. Tudzynski
  • 通讯作者:
    J. Schumacher;L. Studt;P. Tudzynski
Regulation of conidiation in Botrytis cinerea involves the light-responsive transcriptional regulators BcLTF3 and BcREG1
  • DOI:
    10.1007/s00294-017-0692-9
  • 发表时间:
    2017-10-01
  • 期刊:
  • 影响因子:
    2.5
  • 作者:
    Brandhoff, Beate;Simon, Adeline;Schumacher, Julia
  • 通讯作者:
    Schumacher, Julia
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Dr. Julia Schumacher其他文献

Dr. Julia Schumacher的其他文献

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{{ truncateString('Dr. Julia Schumacher', 18)}}的其他基金

Study of incompatible Arabidopsis thaliana-Botrytis cinerea interactions to identify plant responses that may lead to increased resistance to the pathogen
研究不相容的拟南芥-灰霉病相互作用,以确定可能导致增强对病原体抗性的植物反应
  • 批准号:
    222121053
  • 财政年份:
    2012
  • 资助金额:
    --
  • 项目类别:
    Research Fellowships

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