Central proteomic platform for the Research Unit

该中心项目管理和执行明斯特大学植物生物化学和生物技术研究所蛋白质组平台提供的服务。在第一和第二资助期，已经建立并进行了体外和体内样品的磷酸化蛋白质组学分析。蛋白质组中心项目将继续作为研究小组进行肽和蛋白质鉴定、磷酸肽和蛋白质分析以及比较蛋白质组学的平台。研究单位内的各个研究小组将在蛋白水解消化和质谱分析之前进行蛋白质样品制备。中央项目将在这方面发挥咨询作用。蛋白质组平台将继续对样品进行蛋白水解（胰蛋白酶）消化和进一步的样品处理，即通过 SIMAC 和 ERLIC-TiO2 策略富集磷酸肽以及质谱分析。体内拟南芥样品的处理和质谱分析将是第三个资助期的中心。由于新型 Q-Exactive plus 质谱仪的资助出现，新的重点将放在复杂植物样品中感兴趣的肽和蛋白质磷酸化的定量上。

This central Project administers and performs the services provided by the proteomic platform at the Institute for Biochemistry and Biotechnology of Plants at the University of Münster. In the first and second funding period phosphoproteomic analyses from in vitro and in vivo samples have been established and conducted. The proteomic central project will continue to serve as a platform for peptide and protein identification, analysis of phospho-peptides and ¿proteins and comparative proteomics for the research group. Individual research groups within the Research Unit will perform protein sample preparations prior to proteolytic digestion and mass spectrometric analysis. The central project will have an advisory function in this respect. The proteomic platform will continue to perform proteolytic (tryptic) digestion of the sample and further sample handling, i.e. enrichment of phosphopeptides via SIMAC and ERLIC-TiO2 strategies, and mass spectrometric analyses. The handling and mass spectrometric analyses of in vivo Arabidopsis samples will be in the center of the third funding period. Due to the advent of funding of a new Q-Exactive plus mass spectrometer, a new focus will be put on quantitation of peptide and protein phosphorylation of interest in complex plant samples.