Molecular physiology of synaptic vesicle recycling at the hair cell ribbon synapse (A08*)

毛细胞带突触突触小泡回收的分子生理学 (A08*)

• 批准号: 269009167
• 金额: --
• 依托单位: Institut für Auditorische Neurowissenschaften
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2017-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/269009167?language=en
• 关键词: Molecular; physiology; synaptic; vesicle; recycling

In order to sustain high rates of synaptic transmission, inner hair cells (IHCs) need to efficiently recycle synaptic vesicles. Yet, little is known about the mechanisms of SV recycling at IHC ribbon synapses. Here we plan to test the hypotheses that in IHCs: 1) AP-2 contributes to exocytosis-endocytosis coupling and/or clathrin coat assembly at the plasma membrane and endosomal-like vacuoles; 2) dynamins operate in fission during endocytic membrane retrieval and during vesicle reformation from endosomal cisterns; and 3) endophilins as well as synaptojanin mediate uncoating of clathrin-coated vesicles. To achieve this we will carry out morphological and functional studies of IHC synapses in relevant mouse mutants

为了维持高速率的突触传递，内毛细胞(IHC)需要有效地回收突触小泡。然而，对IHC带状突触中SV循环的机制知之甚少。在这里，我们计划测试在IHC中的假设：1)AP-2有助于胞吐-内吞偶联和/或在质膜和内体样空泡处组装笼状蛋白涂层；2)动力蛋白在内细胞膜回收和内体囊泡重塑过程中进行分裂；以及3)内嗜性蛋白和突触素介导笼状蛋白包裹的囊泡的去涂层。为了实现这一点，我们将对相关小鼠突变体的IHC突触进行形态和功能研究