Role of mitochondrial Telomerase Reverse Transcriptase and mitochondrial p27 in myocardial infarction (A04)

我们将利用重组细胞穿透线粒体靶向p27，研究线粒体p27在I/R后早期的功能。此外，我们将评估端粒酶激活剂TA-65是否可以改善I/R后的预后。线粒体p27和线粒体TERT的作用也将在致糖尿病饮食的小鼠中进行探讨。此外，我们将证明这两种蛋白质的潜在共同上游调节因子的概念。最后，我们将描述它们的线粒体相互作用组和两种蛋白质对转录组的影响。

We will investigate the functions of mitochondrial p27 in the early phase after I/R in vivo in mice containing p27 exclusively in the mitochondria and by applying a recombinant cell-penetrating mitochondrially targeted p27. Furthermore, we will assess if the telomerase activator TA-65 can improve the outcomes after I/R. The role of mitochondrial p27 and mitochondrial TERT will also be explored in mice on a diabetogenic diet. Furthermore, we will prove the concept of potential common upstream regulators of both proteins. Finally, we will characterize their mitochondrial interactome and the impact of both proteins on the transcriptome.