Establishment of endosomal membrane domains by guanine nucleotide exchange factors (P17)

通过鸟嘌呤核苷酸交换因子建立内体膜结构域（P17）

• 批准号: 269763204
• 金额: --
• 依托单位: Institut für Biochemie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/269763204?language=en
• 关键词: Establishment; endosomal; membrane; domains; guanine

Project P17 investigates how guanine nucleotide exchange factors (GEFs) integrate different cellular cues to properly activate their cognate Rab GTPase in the spatio-temporal coordination of intracellular traffic. The group will elucidate the molecular basis how a particular class of GEFs, LH-GEFs, establish microcompartments by (i) biochemical and structural characterization of regulatory proteins (ii), studying membrane association of LH-GEFs through lipid interactions in vitro and sub-cellular localization in vivo, and (iii) determining the atomic structure and overall architecture of LH-GEFs.

P17项目研究了鸟嘌呤核苷酸交换因子（GEF）如何整合不同的细胞线索，以正确激活其同源Rab GTdR，在细胞内交通的时空协调中。该小组将阐明一个特定类别的GEFs，LH-GEFs，如何建立微区室的分子基础（i）调节蛋白的生化和结构表征（ii），通过体外脂质相互作用和体内亚细胞定位研究LH-GEFs的膜缔合，以及（iii）确定LH-GEFs的原子结构和整体架构。