Establishment of endosomal membrane domains by guanine nucleotide exchange factors (P17)

P17项目研究了鸟嘌呤核苷酸交换因子（gef）如何整合不同的细胞信号，在细胞内交通的时空协调中正确激活其同源Rab GTPase。该小组将通过(i)调节蛋白的生化和结构表征（ii）通过体外脂质相互作用和体内亚细胞定位研究LH-GEFs的膜关联以及（iii）确定LH-GEFs的原子结构和整体结构来阐明一类特定GEFs， LH-GEFs如何建立微室的分子基础。

Project P17 investigates how guanine nucleotide exchange factors (GEFs) integrate different cellular cues to properly activate their cognate Rab GTPase in the spatio-temporal coordination of intracellular traffic. The group will elucidate the molecular basis how a particular class of GEFs, LH-GEFs, establish microcompartments by (i) biochemical and structural characterization of regulatory proteins (ii), studying membrane association of LH-GEFs through lipid interactions in vitro and sub-cellular localization in vivo, and (iii) determining the atomic structure and overall architecture of LH-GEFs.