Development of a Treatment Concept for Dental Pulp Tissue Engineering

牙髓组织工程治疗概念的开发

• 批准号: 270069690
• 负责人: Professorin Dr. Kerstin Galler, Ph.D.
• 金额: --
• 依托单位: Zahnklinik 1 - Zahnerhaltung und Parodontologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/270069690?language=en
• 关键词: Development; Treatment; Concept; Dental; Pulp

Conventional root canal treatment involves the complete removal of inflamed or necrotic pulp tissue from the root canal system, enlargement, disinfection and obturation of the void with a synthetic material. However, the dental pulp, which fulfils several important functions, and thus tooth vitality are lost after such a procedure. Recent advances in stem cell biology and dental pulp tissue engineering as well as clinical reports of regenerative procedures to sustain tooth vitality might be the prelude of a paradigm shift in endodontics and lead to the establishment of treatment strategies enabling us to regenerate dental pulp in the future. Whereas implantation of autologous stem cells is a promising strategy, it is hampered by issues of cell harvest and expansion as well as high cost. Alternatively, cell-free approaches could be considered, which involve the recruitment, migration, proliferation and differentiation of resident stem cells. The conditions for such a regenerative procedure might be optimized by the use of a bioactive scaffold material, which is placed into the root canal, and furthermore by taking advantage of growth factors entrapped in the dentin matrix. Modified protocols for root canal preparation, irrigation and filling will be required in order to provide an environment that is conducive for stem cell recruitment and new tissue formation. In the proposed project, we intend to establish a protocol for a biology-based approach to endodontic treatment. The overall goal of this application is to establish a procedure for dental pulp regeneration, which follows the cell-homing concept. After partial or complete removal of pulp tissue in case of irreversibly inflamed or necrotic tissue, a regenerative procedure will include 1) disinfection of the root canal, 2) release of growth factors by EDTA-conditioning of root canal dentin, 3) collection of irrigation solution with growth factors from the root canal, 4) mixture with a biomaterial capable of growth factor-binding, 5) re-insertion of growth-factor-laden material into the root canal and 6) coverage of the scaffold with a bioactive cement followed by tight sealing of the canal system. This work could contribute to the development of a treatment strategy to engineer dental pulp which does not require the application of exogenous stem cells or growth factors and which might thus be well applicable in regenerative endodontics.

传统的根管治疗包括从根管系统中完全去除发炎或坏死的牙髓组织、扩大、消毒以及用合成材料封闭空隙。然而，在这种手术后，履行多种重要功能的牙髓和牙齿活力就会丧失。干细胞生物学和牙髓组织工程的最新进展以及维持牙齿活力的再生程序的临床报告可能是牙髓学范式转变的前奏，并导致治疗策略的建立，使我们能够在未来再生牙髓。尽管自体干细胞植入是一种很有前途的策略，但它受到细胞收获和扩增以及高成本问题的阻碍。或者，可以考虑无细胞方法，其中涉及驻留干细胞的招募、迁移、增殖和分化。这种再生过程的条件可以通过使用放置在根管中的生物活性支架材料以及进一步利用捕获在牙本质基质中的生长因子来优化。需要修改根管准备、冲洗和填充方案，以提供有利于干细胞募集和新组织形成的环境。在拟议的项目中，我们打算建立一个基于生物学的牙髓治疗方法的协议。该应用的总体目标是建立一种遵循细胞归巢概念的牙髓再生程序。在出现不可逆的发炎或坏死组织的情况下，部分或完全去除牙髓组织后，再生程序将包括 1) 根管消毒，2) 通过根管牙本质的 EDTA 调节释放生长因子，3) 从根管中收集含有生长因子的冲洗液，4) 与能够结合生长因子的生物材料混合，5) 重新插入牙根管。 将含有生长因子的材料放入根管中，6）用生物活性水泥覆盖支架，然后紧密密封根管系统。这项工作可能有助于开发一种治疗策略来设计牙髓，该策略不需要应用外源干细胞或生长因子，因此可能很好地适用于再生牙髓病。

项目成果

Dentine matrix proteins: isolation and effects on human pulp cells

• DOI: 10.1111/iej.12754
• 发表时间: 2018-05
• 期刊: International Endodontic Journal
• 影响因子: 5
• 作者: M. Widbiller;A. Eidt;S. R. Lindner;K. Hiller;H. Schweikl;Wolfgang Buchalla;K. Galler

Ultrasonic activation of irrigants increases growth factor release from human dentine

• DOI: 10.1007/s00784-016-1824-1
• 发表时间: 2017-04-01
• 期刊: CLINICAL ORAL INVESTIGATIONS
• 影响因子: 3.4
• 作者: Widbiller, M.;Eidt, A.;Galler, K. M.
• 通讯作者: Galler, K. M.

Cell Homing for Pulp Tissue Engineering with Endogenous Dentin Matrix Proteins

• DOI: 10.1016/j.joen.2018.02.011
• 发表时间: 2018-06-01
• 期刊: JOURNAL OF ENDODONTICS
• 影响因子: 4.2
• 作者: Widbiller, Matthias;Driesen, Ronald B.;Galler, Kerstin M.
• 通讯作者: Galler, Kerstin M.