Microbial degradation of sulfoquinovose and plant sulfolipids

磺基奎诺糖和植物硫脂的微生物降解

• 批准号: 270766152
• 负责人: Professor Dr. David Schleheck, Ph.D.
• 金额: --
• 依托单位: Arbeitsgruppe Limnische Mikrobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/270766152?language=en
• 关键词: Microbial; degradation; sulfoquinovose; plant; sulfolipids

Sulfoquinovose (SQ; 6-deoxy-6-sulfoglucose) is the polar headgroup of the sulfolipid sulfoquinovosyl diacylgycerol (SQDG) in the photosynthetic membranes of all higher plants, mosses, ferns and algae, and in most photosynthetic bacteria. The global production of SQ by phototrophs has been estimated to comprise app. 10,000,000,000 tons per year. Thus, the degradation and recycling of the sulfur bound in SQDG/SQ in form of inorganic sulfite or sulfate by bacteria, is an important step of the biogeochemical sulfur cycle. However, the study of the SQDG/SQ degradation pathways in bacteria, and of the biochemistry, enzymes and genes involved, has just been started (my preliminary work). The aim of the proposed project is to define the different pathways, intermediates and the enzymes and genes involved in SQ and SGDG utilization by different aerobic and anaerobic bacteria, through enrichment and isolation, genomics, reverse genetics, biochemistry and analytical chemistry.Hence, the project will produce invaluable information on yet unknown biochemistry, intermediates, enzymes and genes, and will provide a more detailed understanding of the functions and roles of microbes in the biogeochemical sulfur cycle.

磺基喹诺糖（SQ; 6-脱氧-6-磺基葡萄糖）是所有高等植物、苔藓、蕨类和藻类以及大多数光合细菌的光合膜中磺基脂质磺基喹诺糖基二酰基甘油（SQDG）的极性头基。据估计，全球光养生物生产的SQ约为每年10，000，000，000吨。因此，细菌对SQDG/SQ中硫的降解和再循环是硫的非地球化学循环的重要步骤。然而，对SQDG/SQ在细菌中的降解途径以及所涉及的生物化学、酶和基因的研究才刚刚开始（我的初步工作）。该项目的目的是通过富集和分离、基因组学、反向遗传学、生物化学和分析化学，确定不同好氧和厌氧细菌利用SQ和SGDG的不同途径、中间体、酶和基因，从而为未知的生物化学、中间体、酶和基因提供宝贵的信息，并将提供一个更详细的了解微生物在地球化学硫循环中的功能和作用。

项目成果

A glycyl radical enzyme enables hydrogen sulfide production by the human intestinal bacterium Bilophila wadsworthia

• DOI: 10.1073/pnas.1815661116
• 发表时间: 2019-02-19
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Peck, Spencer C.;Denger, Karin;Schleheck, David
• 通讯作者: Schleheck, David

Environmental and Intestinal Phylum Firmicutes Bacteria Metabolize the Plant Sugar Sulfoquinovose via a 6-Deoxy-6-sulfofructose Transaldolase Pathway

• DOI: 10.1016/j.isci.2020.101510
• 发表时间: 2020-09-25
• 期刊: ISCIENCE
• 影响因子: 5.8
• 作者: Frommeyer, Benjamin;Fiedler, Alexander W.;Schleheck, David
• 通讯作者: Schleheck, David