The function of the osteocyte network and its influence on the surrounding bone material

骨细胞网络的功能及其对周围骨材料的影响

• 批准号: 272944896
• 负责人: Dr. Wolfgang Wagermaier, Ph.D.
• 金额: --
• 依托单位: Abteilung Biomaterialien
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/272944896?language=en
• 关键词: function; osteocyte; network; its; influence

Bone consists of mineralized tissue, representing a nanostructured organic-inorganic composite material, and cells (osteocytes) distributed in a network-like porous space, the osteocyte lacunar canalicular network (OLCN). Osteocytes are thought to sense mechanical stimuli and, therefore, act as control center for bone renewal (remodeling) and bone adaptation. In addition, evidence increases that osteocytes can actively change the mineral content of the surrounding bone. This process may contribute to mineral homeostasis and change the mechanical properties of bone.Only since recently, the reaction of bone to a controlled mechanical stimulus can be monitored by multiple imaging. In these samples both the mechanical stimulation and the recent history of remodeling events is known. Over the last years, we have developed methods to image and quantify the OLCN in bone and have a long term experience in characterizing the organic and inorganic phases of bone using X-ray scattering and Raman spectroscopy. Applying these technological advances to bone samples of known structural changes, we want to test the hypothesis, if the osteocyte network acts as a mechanosensing system as well as a transport system during (de)mineralization processes. To test this hypothesis we first characterize in the same bone sample the organization of the cellular component (i.e., the structure of the OLCN) and the material component (i.e., the mineral particles and organic collagen matrix) and then analyze spatial correlations between them. Our specific aims are: 1) To find evidence that the OLCN adapts its topology to local mechanical strains. 2) To detect influences of the OLCN on characteristics of the bone material, in particular, in the vicinity of remodeling sites. 3) To correlate transport-parameters from the OLCN with remodeling and mineralization patterns 4) To quantify bone mineral quality and OLCN topology in regions with distinct order of tissue organization. To tackle the last objective, we study as a second model system bone healing in a mouse femur. During healing a so-called fracture callus is formed rapidly with different order of tissue organization. Since the temporal course of the callus formation is known this model system allows a closer look at the formation of the OLCN and its interaction with the surrounding material.Our approach for this project is that a pure biological analysis of osteocytes and their communication with other cells is insufficient for an understanding of their role for bone health. Our materials science viewpoint will contribute to a clarification of the interaction of the OLCN with the surrounding environment in bones where the recent structural changes (remodeling, healing) are known.

骨由矿化组织和细胞组成，矿化组织代表纳米结构的有机-无机复合材料，细胞（骨细胞）分布在网状多孔空间中，即骨细胞腔隙小管网络（OLCN）。骨细胞被认为能够感知机械刺激，因此，骨细胞是骨更新（重塑）和骨适应的控制中心。此外，越来越多的证据表明，骨细胞可以主动改变周围骨骼的矿物质含量。这一过程可能有助于矿物质的稳态和改变骨的机械性能。直到最近，骨对受控机械刺激的反应才能通过多重成像来监测。在这些样本中，机械刺激和重塑事件的近期历史都是已知的。在过去的几年里，我们已经开发出了成像和量化骨中OLCN的方法，并且在使用X射线散射和拉曼光谱表征骨的有机和无机相方面具有长期的经验。将这些技术进步应用于已知结构变化的骨样品，我们想验证这一假设，如果骨细胞网络在矿化（脱）过程中充当机械传感系统以及运输系统。为了检验这一假设，我们首先在相同的骨样品中表征细胞组分的组织（即，OLCN的结构）和材料组分（即，矿物颗粒和有机胶原基质），然后分析它们之间的空间相关性。我们的具体目标是：1）找到OLCN适应局部机械应变的证据。2)检测OLCN对骨材料特性的影响，特别是在重塑部位附近。3)将来自OLCN的转运参数与重塑和矿化模式相关联4）量化具有不同组织结构顺序的区域中的骨矿物质质量和OLCN拓扑结构。为了解决最后一个目标，我们研究作为第二个模型系统在小鼠股骨骨愈合。在愈合过程中，所谓的骨折骨痂迅速形成，具有不同的组织结构。由于骨痂形成的时间过程是已知的，该模型系统允许更仔细地观察OLCN的形成及其与周围物质的相互作用，我们对该项目的方法是，骨细胞及其与其他细胞的通信的纯生物学分析是不足以理解它们对骨骼健康的作用。我们的材料科学观点将有助于阐明OLCN与骨骼周围环境的相互作用，其中最近的结构变化（重塑，愈合）是已知的。

项目成果

Network architecture strongly influences the fluid flow pattern through the lacunocanalicular network in human osteons

• DOI: 10.1007/s10237-019-01250-1
• 发表时间: 2019-11-28
• 期刊: BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
• 影响因子: 3.5
• 作者: van Tol, Alexander F.;Roschger, A.;Weinkamer, Richard
• 通讯作者: Weinkamer, Richard

Function of the Osteocyte Lacunocanalicular Network in Bone Mechanoresponsiveness

骨细胞腔隙小管网络在骨力学反应中的功能

• DOI: 10.18452/22768
• 发表时间: 2021
• 作者: van Tol

The contribution of the pericanalicular matrix to mineral content in human osteonal bone.

• DOI: 10.1016/j.bone.2019.03.018
• 发表时间: 2019-06
• 期刊: Bone
• 影响因子: 4.1
• 作者: A. Roschger;P. Roschger;W. Wagermaier;J. Chen;A. V. van Tol;F. Repp;S. Blouin;A. Berzlanovich;G. Gruber;K. Klaushofer;P. Fratzl;R. Weinkamer