RNA structure elucidation using site-specific dynamic nuclear polarization in the solid state (A10*)

利用魔角自旋（MAS）核磁共振技术对RNA进行定点动态核极化（DNP）是一种很有潜力的结构信息提取方法。我们将研究几种技术的分子内标记的RNA与顺磁性极化剂，包括取代天然结合的Mg2+与Mn2+，顺磁性标记的配体的结合，和基于金属螯合物的顺磁性标签的定点掺入。这使我们能够同时提取多个核的中远程距离约束，并将生物NMR的工具箱扩展到使用常规NMR无法有效研究的系统

Site-specific dynamic nuclear polarization (DNP) on RNA is a potential method for extracting structural information using magic-angle spinning (MAS) NMR. We will investigate several techniques for intramolecular labeling of RNA with paramagnetic polarizing agents, including substitution of natively bound Mg2+ with Mn2+, binding of paramagnetically labeled ligands, and site-directed incorporation of paramagnetic tags based on metal chelates. This allows us to extract medium- to long-range distance constraints for multiple nuclei at once and extend the toolbox of biological NMR towards systems which cannot be effectively investigated using conventional NMR