RNA structure elucidation using site-specific dynamic nuclear polarization in the solid state (A10*)

利用幻角旋转(MAS)核磁共振技术提取结构信息是一种很有潜力的方法。我们将研究几种用顺磁极化剂标记RNA的技术，包括用Mn2取代天然结合的Mg2，结合顺磁标记的配体，以及基于金属螯合物的顺磁标记的定点掺入。这使得我们可以一次提取多个原子核的中长程距离约束，并将生物核磁共振工具箱扩展到使用常规核磁共振无法有效研究的系统

Site-specific dynamic nuclear polarization (DNP) on RNA is a potential method for extracting structural information using magic-angle spinning (MAS) NMR. We will investigate several techniques for intramolecular labeling of RNA with paramagnetic polarizing agents, including substitution of natively bound Mg2+ with Mn2+, binding of paramagnetically labeled ligands, and site-directed incorporation of paramagnetic tags based on metal chelates. This allows us to extract medium- to long-range distance constraints for multiple nuclei at once and extend the toolbox of biological NMR towards systems which cannot be effectively investigated using conventional NMR