Analytische Ultrazentrifuge

分析超速离心法(AUC)是一种分析蛋白质和蛋白质复合体的有力工具。蛋白质在溶液中分析时不需要支持基质。AUC可以准确测定蛋白质的天然相对分子质量、复合亚单位的化学计量、复合体的组装和结合以及蛋白质的构象和形状。与其他方法相比，AUC需要的材料很少，并且可以在广泛的分子量和结合常数范围内表征蛋白质(络合物)。所应用的机器提供了使用吸光度和瑞利干涉光学的双重检测系统。干涉光学允许快速的扫描速度，并且产生比单独的吸收光学要详细得多的数据。它们还允许对浓缩和非吸收样品进行准确检测。

Analytical ultracentrifugation (AUC) is a powerful tool to characterize proteins and protein complexes.Proteins are analyzed in solution without a supporting matrix. AUC allows for the accurate determination of protein native molecular mass, complex subunit stoichiometry, complex assembly and associations, as well as protein conformation and shape. AUC requires little material compared to other methods and allows characterization of proteins (complexes) over a wide range of molecular weights and association constants.The machine applied offers a double detection system using absorbance and Rayleigh interference optics. Interference optics allow for rapid scan speeds and produce much more detailed data than absorbance optics alone. They also allow for the accurate detection of concentrated as well as non-absorbing samples.