Analytische Ultrazentrifuge

分析超速离心机

• 批准号: 274617267
• 金额: --
• 依托单位: Ludwig-Maximilians-Universität München (LMU)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/274617267?language=en
• 关键词: Analytische; Ultrazentrifuge

Analytical ultracentrifugation (AUC) is a powerful tool to characterize proteins and protein complexes.Proteins are analyzed in solution without a supporting matrix. AUC allows for the accurate determination of protein native molecular mass, complex subunit stoichiometry, complex assembly and associations, as well as protein conformation and shape. AUC requires little material compared to other methods and allows characterization of proteins (complexes) over a wide range of molecular weights and association constants.The machine applied offers a double detection system using absorbance and Rayleigh interference optics. Interference optics allow for rapid scan speeds and produce much more detailed data than absorbance optics alone. They also allow for the accurate detection of concentrated as well as non-absorbing samples.

分析超滤（AUC）是一种表征蛋白质和蛋白质复合物的强有力工具，蛋白质在溶液中分析时无需支持基质。AUC允许准确测定蛋白质天然分子量、复合物亚基化学计量、复合物组装和缔合以及蛋白质构象和形状。与其他方法相比，AUC需要的材料很少，并且可以在广泛的分子量和缔合常数范围内表征蛋白质（复合物）。所应用的机器提供了使用吸光度和瑞利干涉光学的双重检测系统。干涉光学器件允许快速扫描速度，并产生比单独的吸收光学器件更详细的数据。它们还可以准确检测浓缩和非吸收样品。