Analytische Ultrazentrifuge

分析超速离心机

• 批准号: 274617267
• 金额: --
• 依托单位: Ludwig-Maximilians-Universität München (LMU)
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/274617267?language=en
• 关键词: Analytische; Ultrazentrifuge

Analytical ultracentrifugation (AUC) is a powerful tool to characterize proteins and protein complexes.Proteins are analyzed in solution without a supporting matrix. AUC allows for the accurate determination of protein native molecular mass, complex subunit stoichiometry, complex assembly and associations, as well as protein conformation and shape. AUC requires little material compared to other methods and allows characterization of proteins (complexes) over a wide range of molecular weights and association constants.The machine applied offers a double detection system using absorbance and Rayleigh interference optics. Interference optics allow for rapid scan speeds and produce much more detailed data than absorbance optics alone. They also allow for the accurate detection of concentrated as well as non-absorbing samples.

分析性超速离心（AUC）是表征蛋白质和蛋白质复合物的强大工具。在没有支持基质的无溶液中分析蛋白质。 AUC允许准确测定蛋白质天然分子质量，复杂的亚基化学计量，复杂的组装和关联以及蛋白质构象和形状。与其他方法相比，AUC几乎不需要材料，并且可以在各种分子量和关联常数上表征蛋白质（配合物）。机器应用的机器使用吸光度和雷利干扰光学器件提供了双重检测系统。干涉光学元件可以快速扫描速度并产生比仅吸光度光学元件更详细的数据。它们还允许准确检测浓缩和非吸收样品。