Methyl group transfer in biological pathways via the 22nd proteinogenic amino acid pyrrolysine (A10*)

第22位氨基酸吡咯菌素构成了甲基转移酶的催化中心，在甲烷肉毒科古老的小型家族中。在这个体系中，甲基从单乙胺、二乙胺或三乙胺底物转移而不需要额外的辅因子。在即将到来的资助期，将分析甲基营养甲烷化所需蛋白质的结构和功能。该项目的主要目标将是获得对氮代谢发展的新见解，氮代谢动态和机械地依赖于底物三甲胺和甘氨酸甜菜碱。

The 22nd amino acid pyrrolysin constitutes the catalytic centre of methyltransferases in the small archaeal family of Methanosarcinaceae. In this system, methyl group transfer from mono-, di- or triethylamine substrates is achieved without the need for an additional cofactor. In the upcoming funding period, the structure and function of proteins required for methylotrophic methanogenesis will be analysed. The main goal of this project will be to obtain novel insights into the development of a nitrogen metabolism which is dynamically and mechanistically dependent on the substrates trimethylamine and glycine betaine.