Elucidation of supportive microRNAs during hepatic specification of reprogrammed cells in liver cell therapy (C12*)

阐明肝细胞治疗中重编程细胞的肝脏规范期间的支持性 microRNA (C12*)

• 批准号: 278269906
• 金额: --
• 依托单位: Klinik für Gastroenterologie, Hepatologie und Endokrinologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/278269906?language=en
• 关键词: Elucidation; supportive; microRNAs; during; hepatic

The new project C12 uses an alternative approach to generate sufficient numbers of autologous hepatocytes from stem cell sources. An advantage of using autologous liver cells is that major immunosuppressive therapy could be avoided in this setting. The group has already identified several microRNAs that support hepatocyte differentiation from pluripotent stem cells in a DFG-funded project. They will now continue to use combinations of the miRs identified in their earlier work. In addition, they will screen hepatoma cells in order to identify the mRNA targets of the miRs. Transdifferentiation of fibroblasts directly into induced hepatic cells (iHeps) represents another potential source of mature hepatocytes as recently discovered by two groups. C12 will also investigate the potential of microRNAs to facilitate this direct transdifferentiation.

新项目C12使用另一种方法从干细胞来源产生足够数量的自体肝细胞。使用自体肝细胞的一个优点是，在这种情况下可以避免主要的免疫抑制治疗。在DFG资助的一个项目中，该小组已经确定了几种支持从多能干细胞分化为肝细胞的microRNA。他们现在将继续使用他们先前工作中确定的多指标指标的组合。此外，他们还将筛选肝癌细胞，以确定miRs的mRNA靶点。两个小组最近发现，成纤维细胞直接转分化为诱导肝细胞(IHEPS)是另一个潜在的成熟肝细胞来源。C12还将研究microRNAs促进这种直接转分化的潜力。