Spatiotemporal organization of peptidoglycan synthesis and cell division (N) (A17*)

酰基沉积肽抗生素（ADEP）激活细菌肽酶ClpP降解细胞分裂介质FtsZ。利用ADEP作为FtsZ消耗和细胞分裂抑制的工具，我们研究了枯草芽孢杆菌细胞分裂和肽聚糖合成的时空调控方面。在ADEP存在的情况下，杆状细胞形成长丝，其中几乎完全包含侧向肽聚糖，用于多肽组成和转换过程的分析。在去除ADEP后，延长的细胞重新建立了ftsz环，使我们能够表征优先放置ftsz环的位置。

Acyldepsipeptide antibiotics (ADEP) activate bacterial peptidase ClpP to degrade the cell division mediator FtsZ. Using ADEP as a tool for FtsZ depletion and cell division inhibition, we study aspects of spatiotemporal regulation of cell division and peptidoglycan synthesis in Bacillus subtilis. In the presence of ADEP, the rod-shaped cells form long filaments that contain almost exclusively lateral peptidoglycan for analyses of muropeptide composition and turnover processes. After ADEP removal the elongated cells re-establish FtsZ-rings, allowing us to characterize the sites of preferential FtsZ-ring placement.