Elucidating assembly, ligand binding and gating of heterotetrameric CNG channels by FRET (B01)

环核苷酸门控通道(CNG)是光转导通路的最后一步。在这个项目中，光学和电生理技术将被用来阐明激活通道的基本步骤，例如与单个亚基结合的配体序列及其构象重排。该项目的另一部分将集中在细胞确保通道由固定化学计量比的亚基组装的机制上。

Cyclic nucleotide-gated channels (CNG) constitute the last step of the phototransduction cascade. In this project optical and electrophysiological techniques will be used to elucidate elementary steps in the activation of the channel, such as the sequence of ligand binding to the individual subunits and their conformational rearrangement. Another part of the project will focus on the mechanisms by which the cell ensures that the channels are assembled of subunits in a fixed stoichiometry.