Elucidating assembly, ligand binding and gating of heterotetrameric CNG channels by FRET (B01)

通过 FRET 阐明异四聚体 CNG 通道的组装、配体结合和门控 (B01)

• 批准号: 279209550
• 金额: --
• 依托单位: Arbeitsgruppe Biomolekulare Photonik
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/279209550?language=en
• 关键词: Elucidating; assembly; ligand; binding; gating

Cyclic nucleotide-gated channels (CNG) constitute the last step of the phototransduction cascade. In this project optical and electrophysiological techniques will be used to elucidate elementary steps in the activation of the channel, such as the sequence of ligand binding to the individual subunits and their conformational rearrangement. Another part of the project will focus on the mechanisms by which the cell ensures that the channels are assembled of subunits in a fixed stoichiometry.

环核苷酸门控通道（CNG）构成光转导级联的最后一步。在这个项目中，光学和电生理技术将被用来阐明在激活的通道，如配体结合到各个亚基和它们的构象重排的序列的基本步骤。该项目的另一部分将集中在细胞确保通道以固定化学计量组装亚基的机制上。