Understanding kleptoplasty in Sacoglossa (Heterobranchia, Gastropoda) - a cellular histochemical and genomic approach
了解 Sacoglossa(异鳃纲、腹足纲)的盗贼成形术 - 一种细胞组织化学和基因组方法
基本信息
- 批准号:280302282
- 负责人:
- 金额:--
- 依托单位:
- 依托单位国家:德国
- 项目类别:Research Grants
- 财政年份:2015
- 资助国家:德国
- 起止时间:2014-12-31 至 2019-12-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Sacoglossa is an enigmatic heterobranch group, well known for some members ability to incorporate chloroplasts (cps) from their algal food and to survive weeks and months without additionally feeding. Recent transcriptome analyses reject old hypotheses, which suggested a horizontal gene transfer between the algal nuclear genome and the slugs nuclear genome as the basis for this interaction. Thus, the retention of cps cannot be explained by genetic support via gene transfer. New hypotheses must therefore shift to interactions and specific properties of cps and slugs. Our knowledge on Sacoglossa has increased considerably in the last years, including information on phylogenetic relationships, on food specificity, slug behaviour and photosynthetic capability. This information explains several factors that enhance cps maintenance and slugs survival, however only few studies have dealt with the properties on cellular level. Former results (including our own) indicate a specific survival of only few chloroplast types, a time specific survival of cps dependant on starvation and food availability, as well as slugs ontogenetic phase. This project aims to clarify the cp incorporation processon cellular level and explore the role of the cps in the slugs digestive gland cells, especially concerning their contribution as a long-term energetic storage device with the ability to increase nutritional value over a certain period of time. This investigation will utilize ultrastructural, immunohistochemical and confocal laser microscopical methods to determine the path each incorporated cp takes once ingested. Quantitative methods (morphometry, quantitative real time PCR (qPCR) and biochemical analyses) will help to elucidate the contribution of the cps as nutrional depots. Study of gene expression will help to understand physiological and genetic properties of the slugs during various starvation phases. We focus on sister-taxa, Elysia timida and E. cornigera, both of which feed on Acetabularia. E. timida can retain functional cps for months whereas E. cornigera can only retain cps for a few weeks
Sacoglossa是一种神秘的异枝类群,一些成员能够从藻类食物中吸收叶绿体(cps),并且在不额外喂食的情况下存活数周甚至数月。最近的转录组分析拒绝了旧的假设,该假设认为藻类核基因组和鼻涕虫核基因组之间的水平基因转移是这种相互作用的基础。因此,cps的保留不能通过基因转移的遗传支持来解释。因此,新的假设必须转向cps和鼻涕虫的相互作用和特定属性。在过去几年中,我们对Sacoglossa的了解有了相当大的增长,包括系统发育关系、食物特异性、蛞蝓行为和光合能力方面的信息。这一信息解释了提高cps维持和鼻涕虫存活的几个因素,然而只有很少的研究涉及细胞水平的特性。以前的结果(包括我们自己的结果)表明,只有少数叶绿体类型的特定存活率,cps的特定存活率取决于饥饿和食物供应,以及鼻涕虫的个体发生阶段。本项目旨在阐明cp在细胞水平上的整合过程,并探讨cps在鼻涕虫消化腺细胞中的作用,特别是它们作为一种长期能量储存装置的贡献,并能在一定时间内增加营养价值。本研究将利用超微结构,免疫组织化学和共聚焦激光显微镜方法来确定每个合并的cp一旦摄入的路径。定量方法(形态计量学、定量实时PCR和生化分析)将有助于阐明cps作为营养仓库的贡献。基因表达的研究将有助于了解蛞蝓在不同饥饿阶段的生理和遗传特性。我们重点研究了姐妹类群,Elysia timida和E. cornigera,它们都以髋臼为食。野田菊可以保留几个月的功能cps,而野田菊只能保留几个星期的功能cps
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Examining the retention of functional kleptoplasts and digestive activity in sacoglossan sea slugs
- DOI:10.1007/s13127-016-0308-0
- 发表时间:2017-03-01
- 期刊:
- 影响因子:1.6
- 作者:Laetz, Elise M. J.;Ruehr, Peter T.;Waegele, Heike
- 通讯作者:Waegele, Heike
Comparing amylose production in two solar-powered sea slugs: the sister taxa Elysia timida and E. cornigera (Heterobranchia: Sacoglossa)
比较两种太阳能海蛞蝓的直链淀粉产量:姊妹类群 Elysia timida 和 E cornigera(异鳃纲:Sacoglossa)
- DOI:10.1093/mollus/eyy047
- 发表时间:2018
- 期刊:
- 影响因子:1.2
- 作者:Laetz EMJ;Wägele H
- 通讯作者:Wägele H
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Professor Dr. Thomas Bartolomaeus其他文献
Professor Dr. Thomas Bartolomaeus的其他文献
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{{ truncateString('Professor Dr. Thomas Bartolomaeus', 18)}}的其他基金
Ultrastrukturelle und immunhistochemische Untersuchungen der Organogenese bei Nemertea zur Klärung ihrer Stellung im System der Metazoa
对纽虫器官发生的超微结构和免疫组织化学研究,以阐明其在后生动物系统中的地位
- 批准号:
24165214 - 财政年份:2006
- 资助金额:
-- - 项目类别:
Research Grants
Ultrastructural studies into nephridial development and embryonic coelom fate in arthropods III: reconstructionof ancestral states
节肢动物肾发育和胚胎体腔命运的超微结构研究 III:祖先状态的重建
- 批准号:
5452179 - 财政年份:2005
- 资助金额:
-- - 项目类别:
Priority Programmes
Ultrastrukturelle Untersuchungen zur Nephridialentwicklung und zum Schicksal der embryonalen Coelom-Räume bei Onychophora
甲藻肾发育和胚胎体腔空间命运的超微结构研究
- 批准号:
5400403 - 财政年份:2003
- 资助金额:
-- - 项目类别:
Research Grants
Ultrastruktur der transitorischen Nephridien von "Oligochaeten" und ihre Bedeutung für die Phylogenie der Clitellata (Annelida)
“寡毛类”短暂性肾的超微结构及其对 Clitellata(Annelida)系统发育的意义
- 批准号:
5384149 - 财政年份:2002
- 资助金额:
-- - 项目类别:
Research Grants
Gemeinsame Gewichtung molekularer und morphologischer Merkmale
分子和形态特征的联合加权
- 批准号:
5287924 - 财政年份:2000
- 资助金额:
-- - 项目类别:
Research Grants
Ultrastruktur, Entwicklung und Phylogenie der Phoronida zur Klärung der basalen Verwandtschaftsbeziehungen innerhalb der Radialia
Phoronida 的超微结构、发育和系统发育,以阐明 Radialia 内的基本关系
- 批准号:
5282930 - 财政年份:2000
- 资助金额:
-- - 项目类别:
Research Grants
Phylogenetische Beziehungen "sedentärer" Polychaeten zur Klärung der Verwandtschaftsbeziehungen innerhalb der Annelida
“定居”多毛动物的系统发育关系,以阐明环节动物内的关系
- 批准号:
5103208 - 财政年份:1998
- 资助金额:
-- - 项目类别:
Research Grants
Evolution und Phylogenie der Brachiopoda zur Ermittlung verwandtschaftlicher Beziehungen der "Tentaculata" innerhalb der Bilateria
腕足动物的进化和系统发育以确定双边对称动物内“触手”的关系
- 批准号:
5203234 - 财政年份:1995
- 资助金额:
-- - 项目类别:
Research Grants
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