Regulatory interplay between autophagosomal and endosomal pathways (A04)

自噬体和内体途径之间的调节相互作用 (A04)

• 批准号: 283290422
• 金额: --
• 依托单位: Institut für Biochemie II
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/283290422?language=en
• 关键词: Regulatory; interplay; between; autophagosomal; endosomal

This project targets proteins that link endosomal pathways and autophagy networks. One prime candidate is PLEKHM1 whose role in regulation of autophagosome-lysosome fusion will be analyzed. In addition, we will systematically screen for Rab GAP proteins that coordinate the core autophagy and membrane trafficking machineries. Functional screening will be performed using the CRISPR/Cas9 technology. Moreover, we will dissect the functional role of the Rab GAP TBC1D5 in autophagy, clathrin-mediated endocytosis, and neu-roendocrine signaling using C. elegans as model organism.

该项目针对连接内体途径和自噬网络的蛋白质。一个主要的候选者是PLEKHM 1，其在自噬体-溶酶体融合的调节中的作用将被分析。此外，我们将系统地筛选协调核心自噬和膜运输机制的Rab GAP蛋白。将使用CRISPR/Cas9技术进行功能筛选。此外，我们将使用C. elegans作为模式生物。