Regulatory interplay between autophagosomal and endosomal pathways (A04)

该项目针对连接内体途径和自噬网络的蛋白质。一个主要的候选者是PLEKHM1，其在自噬体-溶酶体融合调节中的作用将被分析。此外，我们将系统筛选协调核心自噬和膜运输机制的Rab GAP蛋白。使用CRISPR/Cas9技术进行功能筛选。此外，我们将以秀丽隐杆线虫为模型生物，剖析Rab GAP TBC1D5在自噬、网格蛋白介导的内吞作用和神经内分泌信号传导中的功能作用。

This project targets proteins that link endosomal pathways and autophagy networks. One prime candidate is PLEKHM1 whose role in regulation of autophagosome-lysosome fusion will be analyzed. In addition, we will systematically screen for Rab GAP proteins that coordinate the core autophagy and membrane trafficking machineries. Functional screening will be performed using the CRISPR/Cas9 technology. Moreover, we will dissect the functional role of the Rab GAP TBC1D5 in autophagy, clathrin-mediated endocytosis, and neu-roendocrine signaling using C. elegans as model organism.