Simultaneous Electrochemical Detection of miRNAs in Human Body Fluids: Development of an Integrated Multiplexed Microfluidic RNA sensor

人体体液中 miRNA 的同步电化学检测:集成多重微流控 RNA 传感器的开发

基本信息

项目摘要

The present project aims to develop a rapid, reliable, and ultrasensitive electrochemical MicroRNA (miRNA) lab-on-a-chip (LOC) detection system with multiplexed microfluidics to gauge different miRNAs in clinically relevant samples (including but not limited to blood plasma, saliva, urine, serum or even whole blood) early, easy and simultaneously. Furthermore, it is dedicated to the discovery of different miRNAs as potential biomarkers for lung tumors and interstitial lung diseases (ILD), which can be employed later for diagnostic and prognostic purposes in the clinical settings, included in such a chip-based microfluidic platform.The early detection of disease-causing agents plays a key role in the clinical medicine and will increase the survival chances of the patients. MiRNAs are small RNA molecules that play a key regulatory role in the regulation of gene expression. Some miRNAs are expressed in a disease-specific manner and can be detected in either increased or decreased concentration.Therefore, miRNAs are gaining increasingly importance as biomarkers for cancer, cardiovascular disease and interstitial lung diseases. During the last decade, it has been demonstrated that miRNAs are well preserved in clinically relevant samples (e.g. blood plasma, serum, saliva and urine) and show considerably higher sensitivity than protein-based biomarkers. These aspects will favor miRNAs based detection systems as a standard in the future diagnostics devices and will allow us to use other samples like urine, or saliva and/or to work with low sample volumes for the detection.Miniaturized diagnostic system devices offer an acceleration of the specific and sensitive detection of various biomarkers, which can be adapted into many areas of medicine - from cheaper clinical trials, to early diagnosis and treatment of complex diseases. The combination of microfluidics and fabrication suitable for mass production allows for the construction of low-cost and portable point of care (POC) diagnostic systems which results in additional advantages in terms of speed, and low sample consumption.The new miRNA detection method realized by this proposal using a unique combination of different research areas (Microtechnology, microfluidics and biomedicine) will assist diagnosis and therapy guiding in ILD. The successful demonstration of the proposed miRNA detection system will provide the advantage that miRNA can be measured directly without amplification. The proof of principle measurements show very promising results and let us motivate to combine the existing immunosensors with different amplifications approaches to achieve a miRNA profiling platform without expensive PCR amplification step.
本项目旨在开发一种快速、可靠、超灵敏的电化学miRNA (miRNA)芯片实验室(lab-on-a-chip, LOC)多路微流体检测系统,以早期、简便、同时检测临床相关样品(包括但不限于血浆、唾液、尿液、血清甚至全血)中的不同miRNA。此外,它致力于发现不同的mirna作为肺肿瘤和间质性肺疾病(ILD)的潜在生物标志物,这些mirna可以在临床环境中用于诊断和预后目的,包括在这样一个基于芯片的微流控平台中。早期发现致病因子在临床医学中起着至关重要的作用,将增加患者的生存机会。mirna是一种小RNA分子,在基因表达调控中起着关键的调节作用。一些mirna以疾病特异性的方式表达,可以在浓度升高或降低时检测到。因此,mirna作为癌症、心血管疾病和间质性肺疾病的生物标志物越来越重要。在过去十年中,已经证明mirna在临床相关样品(如血浆、血清、唾液和尿液)中保存良好,并且显示出比基于蛋白质的生物标志物高得多的敏感性。这些方面将有利于基于mirna的检测系统作为未来诊断设备的标准,并允许我们使用其他样品,如尿液,唾液和/或使用低样本量进行检测。小型化的诊断系统设备加速了对各种生物标志物的特异性和敏感性检测,可以应用于许多医学领域——从更便宜的临床试验到复杂疾病的早期诊断和治疗。微流体和适合大规模生产的制造的结合允许低成本和便携式护理点(POC)诊断系统的构建,这在速度和低样品消耗方面具有额外的优势。该方案实现的新型miRNA检测方法结合了不同的研究领域(微技术、微流体和生物医学),将有助于ILD的诊断和治疗指导。所提出的miRNA检测系统的成功演示将提供miRNA可以直接测量而无需扩增的优势。原理测量的证明显示出非常有希望的结果,并激励我们将现有的免疫传感器与不同的扩增方法相结合,以实现无需昂贵的PCR扩增步骤的miRNA分析平台。

项目成果

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Professor Dr.-Ing. Gerald A. Urban其他文献

Professor Dr.-Ing. Gerald A. Urban的其他文献

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{{ truncateString('Professor Dr.-Ing. Gerald A. Urban', 18)}}的其他基金

µROX - µRNA Redox cycling
µROX - µRNA 氧化还原循环
  • 批准号:
    323700825
  • 财政年份:
    2016
  • 资助金额:
    --
  • 项目类别:
    Research Grants

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Towards Ultrasensitive Detection of Bacterial Extracellular Electron Transfer in Human Gut by Novel Functionalized Carbon Nanotube Electrode Interfaces and Organic Microbial Electrochemical Transistor
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