Aquaporin channels in cell migration

细胞迁移中的水通道蛋白通道

• 批准号: DP160104641
• 负责人: Prof Andrea Yool
• 金额: $ 24.34万
• 依托单位: The University of Adelaide
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-03-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160104641
• 关键词: Aquaporin; channels; cell; migration

The project aims to determine the role of Aquaporin1 (AQP1) in enhancing rapid cell motility. Cell migration is important for development, repair, and protection in multicellular organisms. AQP1 is increased in some rapidly migrating cell types. Loss of AQP1 impairs migration, which is restored by reintroduction of AQP1 but not AQP4. Expected outcomes include defining the features of AQP1 that confer enhanced cell migration. The project will test the hypothesis that dual water and ion channel functions of AQP1 are needed for movement, using migration assays in cells with wild type and mutant AQP1, and selective pharmacological agents developed by the project team to dissect the essential channel properties that enable rapid migration in cancer and stem cells. The project seeks to build knowledge of AQP roles in development, regeneration and surveillance, potentially improving health care by revealing pathways in migration disorders such as metastasis.

该项目旨在确定水通道蛋白1（AQP1）在增强快速细胞运动中的作用。细胞迁移对于多细胞生物的发育、修复和保护是重要的。AQP1在一些快速迁移的细胞类型中增加。AQP1的丢失会损害迁移，而迁移可以通过重新引入AQP1而不是AQP4来恢复。预期的结果包括定义AQP1赋予增强细胞迁移的特征。该项目将测试AQP1的双重水和离子通道功能是运动所必需的假设，使用野生型和突变型AQP1细胞的迁移试验，以及项目团队开发的选择性药理学试剂，以剖析使癌症和干细胞快速迁移的基本通道特性。该项目旨在建立AQP在发育，再生和监测中的作用的知识，通过揭示转移等迁移性疾病的途径，可能改善医疗保健。