Anaerobic Oxidation of Methane in Terrestrial Ecosystems (AOMTE): mechanisms and ecological relevance

陆地生态系统中甲烷的厌氧氧化（AOMTE）：机制和生态相关性

• 批准号: 288187354
• 负责人: Dr. Maxim Dorodnikov
• 金额: --
• 依托单位: Institut für Systematische Botanik und Ökologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2015
• 资助国家: 德国
• 起止时间: 2014-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/288187354?language=en
• 关键词: Anaerobic; Oxidation; Methane; Terrestrial; Ecosystems

Anaerobic oxidation of methane (AOM) is a microbial process of methane (CH4) consumption under anoxic conditions with various terminal electron acceptors (other than oxygen), e.g. sulfate, nitrate, nitrite, some metals (Fe, Mn) or organic compounds. AOM is common in marine ecosystems, where microbial sulfate reduction consumes most of the CH4 produced in sediments. Despite the global significance of AOM, the mechanisms (specific electron acceptors, microbial groups, etc.), optimal conditions and AOM relevance in terrestrial ecosystems are almost unknown. Therefore, there is a strong need for investigation of AOM in terrestrial ecosystems, especially those exposed to prolonged anaerobic conditions such as natural or restored peatlands and rice paddies. This proposal focuses on the AOM mechanisms and intensity within a large climate gradient in four research sites: one natural peatland in Sweden, two restored peatlands in Germany and one rice paddy field in China. To estimate the in situ AOM rate, the belowground 13C-CH4 labeling will be applied. The product of AOM (released 13CO2) together with the dynamics of porewater electron acceptors will allow to assess the AOM intensity and link it to existing environmental conditions at each of the sites. Along with the field studies, the specific mechanisms and the microbial groups driving AOM will be thoroughly investigated in a set of laboratory experiments with the soils from all research sites. In planned microcosms experiments, a broad concentration range of the most common electron acceptors will be added together with 13C-CH4. Application of novel methods of electrochemical analysis (measurement of electron exchange capacities of the electron acceptors), 13C tracing in 13CO2 produced by 13CH4 oxidation and in PLFA and GDGT biomarkers (proxies for bacterial and archaeal communities) will reveal the optimal conditions for the highest potential AOM rates and determine microbial groups responsible for AOM. To our knowledge, the current proposal is the first attempt to estimate the in situ AOM rate and to determine the responsible microbial groups in a range of ecosystems with sustained CH4 production on Eurasian continent. Finally, understanding of AOM mechanisms may change the existing concept of CH4 cycling in terrestrial ecosystems and will improve current process-based models of regional and global carbon balance.

甲烷厌氧氧化(AOM)是甲烷(CH4)在缺氧条件下消耗的微生物过程，末端有各种电子受体(除氧气外)，如硫酸盐、硝酸盐、亚硝酸盐、某些金属(Fe、Mn)或有机化合物。AOM在海洋生态系统中很常见，在那里微生物硫酸盐还原消耗了沉积物中产生的大部分CH4。尽管AOM具有全球意义，但陆地生态系统中AOM的机制(特定电子受体、微生物群等)、最佳条件和AOM相关性几乎是未知的。因此，迫切需要研究陆地生态系统中的AOM，特别是那些暴露在长期厌氧条件下的生态系统，如自然或恢复的泥炭地和稻田。本研究在瑞典的一个天然泥炭地、德国的两个恢复泥炭地和中国的一个水稻田四个研究点，重点研究了大气候梯度下的AOM机制和强度。为了估计原位AOM率，将应用地下13C-CH4标记。AOM的产物(释放的13CO2)与孔隙水电子受体的动态一起，将允许评估AOM的强度，并将其与每个地点的现有环境条件联系起来。随着实地研究，驱动AOM的具体机制和微生物群将在所有研究地点的土壤中进行一系列实验室实验来彻底调查。在计划的微观世界实验中，最常见的电子受体的广泛浓度范围将与13C-CH4一起添加。应用新的电化学分析方法(测量电子受体的电子交换能力)、13CH4氧化产生的13CO2中的13C示踪以及PLFA和GDGT生物标记物(细菌和古生物群落的替代物)将揭示获得最高潜在AOM率的最佳条件，并确定AOM的微生物群。据我们所知，目前的提议是第一次尝试估计原位AOM率，并确定在欧亚大陆持续产生甲烷的一系列生态系统中负责的微生物群。最后，对AOM机制的理解可能会改变陆地生态系统中CH4循环的现有概念，并将改进目前基于过程的区域和全球碳平衡模型。

项目成果

To shake or not to shake: 13C-based evidence on anaerobic methane oxidation in paddy soil

摇动还是不摇动：基于 13C 的稻田厌氧甲烷氧化证据

• DOI: 10.1016/j.soilbio.2019.03.010
• 发表时间: 2019-06
• 期刊: Soil Biology and Biochemistry
• 影响因子: 9.7
• 作者: Lichao Fan;Shahbaz Muhammad;Tida Ge;Jinshui Wu;Dippold Michaela;Thiel Volker;Kuzyakov Yakov;Dorodnikov Maxim
• 通讯作者: Dorodnikov Maxim

Anaerobic oxidation of methane in paddy soil: Role of electron acceptors and fertilization in mitigating CH4 fluxes

水稻土中甲烷的厌氧氧化：电子受体和施肥在缓解 CH4 通量中的作用

• DOI: 10.1016/j.soilbio.2019.107685
• 发表时间: 2020-02-01
• 期刊: SOIL BIOLOGY & BIOCHEMISTRY
• 影响因子: 9.7
• 作者: Fan, Lichao;Dippold, Michaela A.;Dorodnikov, Maxim
• 通讯作者: Dorodnikov, Maxim

To shake or not to shake: Silicone tube approach for incubation studies on CH4 oxidation in submerged soils.

• DOI: 10.1016/j.scitotenv.2018.12.090
• 发表时间: 2019-03
• 期刊: The Science of the total environment
• 作者: Lichao Fan;M. Shahbaz;T. Ge;Jinshui Wu;Y. Kuzyakov;M. Dorodnikov

Active metabolic pathways of anaerobic methane oxidation in paddy soils

• DOI: 10.1016/j.soilbio.2021.108215
• 发表时间: 2021-03-24
• 期刊: SOIL BIOLOGY & BIOCHEMISTRY
• 影响因子: 9.7
• 作者: Fan, Lichao;Schneider, Dominik;Dorodnikov, Maxim
• 通讯作者: Dorodnikov, Maxim