Rapid Test for Antibiotic Resistances in Urological Sample Material by Surface Enhanced Raman Scattering SERS

通过表面增强拉曼散射 SERS 快速检测泌尿样本材料中的抗生素耐药性

• 批准号: 289277506
• 负责人: Professor Dr. Christoph Haisch
• 金额: --
• 依托单位: Lehrstuhl für Analytische Chemie und Wasserchemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/289277506?language=en
• 关键词: Rapid; Test; Antibiotic; Resistances; Urological

In the framework of the project, a combination of surface-enhanced Raman scattering (SERS) with a particular in-situ synthesis method for the SERS-active colloids will be investigated and applied as rapid antibiotic resistance tests. Preliminary studies demonstrate that the specific approach is suitable to reveal within few minutes the efficiency of antibiotics against specific microorganisms on a single organism basis as well as in suspension. As opposed to current approaches, our new method identifies the antibiotic effect of different substances not based on minute changes of the Raman spectrum, but on its effect upon generation and aggregation of the SERS-active colloids. The depolarization of the transmembrane potential by the antimicrobial substance apparently prevents in situ synthesis on the particles´ surface, or the aggregation of the particles there. In consequence, no SERS enhancement takes place and thus, no significant SERS-signal of a dead organism can be detected. This leads to a high significance of the distinction of living and dead organisms, with highly structured and specific signals of the living organisms and essentially a baseline signal for dead organisms. The approach was successfully tested for several E. coli strains. In less than one hour, Polymyxin B-resistant strains could be distinguished from non-resistant strains, with the first significant results available in less than 10 min. The aim of the project is a concise test and validation of the novel approach with respect to applications in urology. Suitable sample preparation as well as data evaluation strategies will be developed, a key focus being set on the compromise between statistical reliability and short analysis time of one to two hours.

在该项目的框架内，将研究表面增强拉曼散射（SERS）与特定的原位合成方法相结合的SERS活性胶体，并将其应用于快速抗生素耐药性测试。初步研究表明，特异性方法适用于在几分钟内揭示抗生素对单个生物体和悬浮液中特定微生物的效率。与目前的方法相反，我们的新方法不是基于拉曼光谱的微小变化来识别不同物质的抗生素作用，而是基于其对sers活性胶体的产生和聚集的影响。抗菌物质对跨膜电位的去极化明显地阻止了颗粒表面的原位合成或颗粒在那里的聚集。因此，没有SERS增强发生，因此，不能检测到死亡生物体的显著SERS信号。这导致了活生物体和死生物体的高度区分，活生物体具有高度结构化和特异性的信号，而死生物体本质上是一个基线信号。该方法已成功地对几种大肠杆菌菌株进行了测试。在不到1小时的时间内，可将多粘菌素b耐药菌株与非耐药菌株区分开来，并在不到10分钟的时间内获得第一个显著结果。该项目的目的是一个简明的测试和验证的新方法，在泌尿外科方面的应用。将制定适当的样品制备和数据评估策略，重点放在统计可靠性和一到两个小时的短分析时间之间的折衷。

项目成果

Identifying Dormant Growth State of Mycobacteria by Orthogonal Analytical Approaches on a Single Cell and Ensemble Basis.

基于单细胞和整体的正交分析方法识别分枝杆菌的休眠生长状态

• DOI: 10.1021/acs.analchem.8b03646
• 发表时间: 2019
• 期刊: Analytical chemistry
• 影响因子: 7.4
• 作者: Neumann;Hoelscher;Haisch;Wieser
• 通讯作者: Wieser

Deuterium uptake in combination with Raman spectroscopy as a tool to investigate antibiotic susceptibility of bacteria

• DOI: 10.1117/12.2553093
• 发表时间: 2020-02
• 作者: D. Bauer;L. Qiu;K. Wieland;Anna-Cathrine Neumann-Cip;A. Wieser;G. Magistro;C. Stief;C. Haisch