Establishing a universal model virus system for breath air aerosol studies, including an appropriate sampling system

建立用于呼吸空气气溶胶研究的通用模型病毒系统，包括适当的采样系统

• 批准号: 468910372
• 负责人: Professor Dr. Christoph Haisch
• 金额: --
• 依托单位: Lehrstuhl für Analytische Chemie und Wasserchemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/468910372?language=en
• 关键词: Establishing; universal; model; virus; system

As stated already in the call, there is a significant lack of reliable data on dispersion, aging, and deposition of Breath Air Aerosol (BAA) carrying viruses. One main reason for this shortcoming is the limited comparability of the numerous studies in the field, which again is caused by the fact that these studies are carried out with a wide range of different particle systems. Some physical properties of BAA can be simulated by polystyrol latex particles, other by nebulizing bacteriophages. These particle systems are only suitable to simulate particle behavior after release. The release process by the human respiratory tract, which is responsible for the distribution of viruses in various (size) fractions and number densities inside BAA particles, is not accessible. However, the release conditions are responsible for the further fate of the particles, such as drying, aging, sedimentation, but also for the infectivity of the enclosed viruses. These effects can only be assessed by clinical tests with patients (e.g. influence or COVID-19 patients), implicating significant health risk as well as a considerable health burden for the patient.The aim of this project is to establish the TT-virus as non-infectious, universal model virus system for studies on viruses in Breath Air Aerosol. The TT virus, named after the initials of the first patient, where it was detected, is a genus of non-enveloped viruses in the family Anelloviridae. Depending on the world region, between 10 and more than 90% of all adults carry this virus, which is considered not to cause any negative health effects. As the TT virus is also contained in the respiratory tract and in BAA, it might be an ideal model organism. The presence of TT viruses in different BAA size fractions will be investigated by means of various sampling and measuring devices, breath air sampling chambers, masks, and helmets available in our labs. Another challenge in virus-carrying aerosol sampling is the low sampling efficiency, particularly when the infectivity of the sampled viruses is to be maintained. Hence, in parallel to the basic investigations on TT viruses on breath air aerosol, a novel sampling device, comprising a water condensation chamber and an impinger, will be constructed to sample viruses contained in BAA with high efficiency and, equally important, in a way that the infectivity is maintained and accessible for further tests.

正如电话会议中已经指出的那样，关于携带病毒的呼气气溶胶(BAA)的扩散、老化和沉积，严重缺乏可靠的数据。这一缺陷的一个主要原因是该领域众多研究的可比性有限，这也是由于这些研究是在广泛的不同粒子系统中进行的事实造成的。BAA的一些物理性质可以通过聚苯乙烯胶乳颗粒来模拟，其他的可以通过雾化噬菌体来模拟。这些粒子系统仅适用于模拟释放后的粒子行为。人类呼吸道的释放过程负责病毒在BAA颗粒内以不同(大小)组分和数密度分布，这是不可访问的。然而，释放条件对颗粒的进一步命运负有责任，如干燥、老化、沉淀，但也对封闭的病毒的传染性负责。这些影响只能通过对患者(例如，影响或新冠肺炎患者)的临床测试来评估，这意味着重大的健康风险以及对患者的相当大的健康负担。本项目的目的是建立TT病毒作为非传染性的通用模型病毒系统，用于呼吸空气气溶胶中的病毒研究。TT病毒以发现它的第一名患者的首字母命名，是Anelloviridae科的一种无包膜病毒属。根据世界地区的不同，10%至90%以上的成年人携带这种病毒，这种病毒被认为不会对健康造成任何负面影响。由于TT病毒也存在于呼吸道和BAA中，它可能是一个理想的模式生物。将通过我们实验室提供的各种采样和测量设备、呼气空气采样室、口罩和头盔来调查不同BAA大小的TT病毒的存在。携带病毒的气溶胶采样的另一个挑战是采样效率低，特别是在要保持采样病毒的传染性的情况下。因此，在对呼气气溶胶中的TT病毒进行基本调查的同时，将建造一种新的采样装置，包括一个水冷凝室和一个撞击器，以高效地对BAA中所含的病毒进行采样，同样重要的是，以一种保持传染性和便于进一步检测的方式对其进行采样。