Investigation of the compositional, positional and functional heterogeneity of MICOS clusters in single mammalian cells (P01)

单个哺乳动物细胞中 MICOS 簇的组成、位置和功能异质性研究（P01）

• 批准号: 289999072
• 金额: --
• 依托单位: Klinik für Neurologie
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/289999072?language=en
• 关键词: Investigation; compositional; positional; functional; heterogeneity

The mitochondrial contact site and cristae organizing system (MICOS) is a large multi-subunit complex in the inner membrane of mitochondria that is required for the maintenance of crista junctions. The MICOS complex is an interaction hub that connects the mitochondrial inner membrane to outer membrane proteins and presumably also to the endoplasmic reticulum (ER). The MICOS clusters exhibit a well-ordered arrangement in the mitochondria. We will investigate to what extent mitochondrial movements as well as fission and fusion dynamics and interactions with cellular structures such as the ER influence the inner-mitochondrial distribution of MICOS. Thereby this project aims to investigate the compositional and functional heterogeneity of MICOS clusters with respect to their sub-mitochondrial localizations using optical nanoscopy and electron microscopy.

线粒体接触位点和嵴组织系统（MICOS）是线粒体内膜中的一个大型多亚基复合体，是维持嵴连接所必需的。MICOS复合物是连接线粒体内膜与外膜蛋白的相互作用中心，并且可能还连接到内质网（ER）。MICOS簇在线粒体中呈现良好的有序排列。我们将研究线粒体运动以及裂变和融合动力学以及与细胞结构（如ER）的相互作用在多大程度上影响MICOS的线粒体内分布。因此，本项目的目的是调查组成和功能的异质性的MICOS集群相对于他们的亚线粒体定位使用光学纳米显微镜和电子显微镜。