Global warming and thermal stress tolerance: assessing the physiological and genetic basis through direct transcriptome sequencing

全球变暖和热应激耐受性：通过直接转录组测序评估生理和遗传基础

• 批准号: 29570682
• 负责人: Professor Dr. Thorsten Reusch, Ph.D.
• 金额: --
• 依托单位: Forschungsbereich 3: Marine Ökologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/29570682?language=en
• 关键词: Global; warming; thermal; stress; tolerance

Prolonged summer heat waves associated with global warming impose increasing stress on coastal communities of temperate European waters that have already resulted in population declines of marine macrophytes. Populations of macroalgae and seagrasses may change their distribution, respond through plasticity, or go extinct. Whether or not local populations possess sufficient genetic variability to survive in the face of global warming through rapid microevolution is currently unknown. The core idea of the proposed project is to experimentally expose replicated genotypes of a `keystone¿ plant, Zostera marina (eelgrass) from several locations differing in thermal regime to summer temperature stress simulating global warming. Fitness related traits (survival, growth, reproduction) are combined with a quantification of transcription levels of a suite of candidate genes that mediate the stress response using real-time polymerase chain reaction. An assessment of additive genetic variance in both, stress gene induction and plant fitness will allow predictions on the potential for microevolution present in local temperate populations; and on the potential of genetic rescue by southern, presumable warm-adapted genotypes. Such studies also serve as starting point for causally relating plant stress tolerance to gene expression polymorphism in the face of global warming.

与全球变暖相关的夏季热浪延长对欧洲温带沃茨的沿海社区造成越来越大的压力，这已经导致海洋大型植物的数量下降。大型藻类和海草的种群可能会改变它们的分布，通过可塑性做出反应，或者灭绝。当地种群是否拥有足够的遗传变异性，通过快速的微进化在全球变暖面前生存，目前尚不清楚。拟议项目的核心思想是实验性地暴露复制基因型的“keystone”植物，Zostera marina（鳗草）从几个不同的位置在热制度的夏季温度应力模拟全球变暖。健身相关的性状（生存，生长，繁殖）相结合的一套候选基因，介导的压力反应，使用实时聚合酶链反应的转录水平的定量。在这两个，胁迫基因诱导和植物健身的加性遗传方差的评估将允许预测的潜力存在于当地温带人群的微进化，并对潜在的南方，假定温暖的适应基因型的遗传救援。这些研究也作为在面对全球变暖时将植物胁迫耐受性与基因表达多态性因果联系起来的起点。