Changes in supramolecular structure of lipid bilayers induced by protein-lipid interactions

蛋白质-脂质相互作用引起的脂质双层超分子结构的变化

• 批准号: 29630552
• 负责人: Privatdozentin Dr. Izabella Brand
• 金额: --
• 依托单位: Institut für Chemie (IfC)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/29630552?language=en
• 关键词: Changes; supramolecular; structure; lipid; bilayers

Structural and conformational changes in lipid bilayers and in proteins taking place during the binding process will be investigated by Polarization Modulation Fourier Transform Infrared Reflection Absorption Spectroscopy (PM FTIRRAS). This research shall open a new way to study the impact of carbohydrate-protein interaction on structure of the membrane. Cholera toxin having resolved by X-ray diffraction crystal structure will serve as the model protein and will be used to verify the applicability of the method. In the further course of this project the interaction of siglec proteins with glycolipids will be studied. Due to the presence of charged lipids and proteins the natural membranes are constantly exposed to high electric fields. Therefore a model lipid bilayer prepared on the electrode surface will provide an excellent environment to understand the role of the electric field in the protein binding. Electrochemical techniques describe only qualitatively the dynamics and structural properties of organic films and proteins involved in binding. The PM FTIRRAS method will allow to understand the processes occurring at the electrified phase boundary at the molecular level. In order to recognize molecular behavior in thin films, electrochemistry will be combined with an in situ spectroscopic technique, sensitive to molecular structure.

将通过偏振调制傅里叶变换红外反射吸收光谱法（PM FTIRRAS）研究结合过程中脂质双层和蛋白质的结构和构象变化。本研究为研究糖蛋白相互作用对膜结构的影响开辟了一条新途径。以经X射线衍射解析晶体结构的霍乱毒素为模型蛋白，验证该方法的适用性。在这个项目的进一步过程中，将研究siglec蛋白与糖脂的相互作用。由于存在带电的脂质和蛋白质，天然膜不断暴露于高电场。因此，在电极表面制备的模型脂质双层将为了解电场在蛋白质结合中的作用提供一个良好的环境。电化学技术仅定性描述了有机膜和参与结合的蛋白质的动力学和结构特性。PM FTIRRAS方法将允许在分子水平上理解在带电相边界处发生的过程。为了识别薄膜中的分子行为，电化学将与对分子结构敏感的原位光谱技术相结合。