Recapitulating Human Brown Adipose Tissue from Pluripotent Stem Cells

点击翻译按钮获取中文摘要

We have established a highly efficient protocol to differentiate hiPSCs to Paraxial mesoderm and Dermomyotome. We also have established an hiPSC MYF5-tdTomato reporter cell line by the Cas9/CRISPR system. Using this cell line, we could achieve MYF5+ BAT progenitor cells and purify the MYF5+ cells by FACS, and then stimulate the brown adipocyte differentiation in SFO3 supplemented with adipose differentiation cocktails. After 80 days of differentiation, cells stored small multilocular lipid droplets. RNA-seq data indicated that the brown adipocyte marker genes PPARα, PGC1α, CEBPα, CEBPβ, PPARγ were highly expressed in these differentiated cells. All of these data indicated that we have successfully established a highly efficient differentiation protocol from hiPSCs to brown adipose tissue.