病態解明と創薬を目標とした妊娠高血圧症候群患者の血小板中microRNA解析

妊娠高血压综合征患者血小板中的 MicroRNA 分析，旨在阐明病理学和药物发现

• 批准号: 21K09061
• 负责人: 金沢 路子
• 金额: $ 2.58万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2025-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K09061/
• 关键词: 妊娠高血圧症症候群; HELLP症候群; 血小板減少症; microRNA発現網羅的解析; 妊娠高血圧症; miRNA

妊娠高血圧症候群、およびその関連疾患であるHELLP（hemolysis, elevated liverenzymes and low platelets）症候群患者にみられる血小板減少症は、経腟分娩や帝王切開術後の弛緩出血による危機的大量出血を引き起こす。近年の研究から、microRNA（miRNA）が妊娠高血圧症候群の病態機序に関与している可能性が示唆されているが、これら疾患群における血小板減少症の詳細な発症メカニズムは、明らかにされておらず、予防法や治療法の開発には至っていない。本研究では、妊娠高血圧症候群、及びHELLP症候群患者を対象に、血小板に発現するmiRNAをターゲットに、次世代シーケンサーを用いて網羅的に配列解析を行い、血小板減少症の発症に関わるmiRNAを同定し、そのメカニズムmiRNAが発現調節を行う遺伝子から、早期診断、及び治療に有用となる標的タンパク質を明らかにし、miRNA核酸医薬の創薬に繋がる結果を得ることを目的とする。現在本研究では、妊娠高血圧症候群およびHELLP症候群患者の血小板分画のmiRNA発現の網羅的解析と正常妊婦の血小板分画のmiRNA発現の網羅的解析を行うために検体を収集し、血小板分画からsmall RNAをmirVanaTM miRNA Isolation Kitを用いて抽出している。HELLP症候群患者については、これまで2件の研究対象者が確保できているが、少なくとも今後数例の検体確保が望ましい状況である。正常妊婦や妊娠高血圧症候群の妊婦の検体はそれぞれ5例以上収集できている。

Patients with hyperemic syndrome of pregnancy, those with hemolysis (elevated liverenzymes and low platelets) syndrome, those with thrombocytopenia syndrome, and those with thrombocytopenia caused by severe bleeding caused by relaxation of the bleeding crisis after the opening of the pregnancy delivery emperor. In recent years, we have studied the pathogenesis of hypertension syndrome in pregnancy, microRNA (miRNA), and the possibility of pregnancy. In recent years, we have studied the pathogenesis and the possibility of pregnancy in patients with high blood syndrome of pregnancy. In this study, patients with hypertension of pregnancy, hypertension of pregnancy, and patients with HELLP syndrome, thrombocytopenia, miRNA syndrome, thrombocytopenia, pregnancy, thrombocytopenia, hypertension, thrombocytopenia, thrombocytopenia, And the results of miRNA nucleic acid therapy were better than those of the control group. Now, in this study, in patients with pregnancy-induced hypertension syndrome (HELLP) and pregnancy-induced hypertension syndrome (HELLP), the analysis of platelet separation in normal pregnancy, platelet separation in pregnancy, miRNA in normal pregnancy, platelet extraction mirVanaTM miRNA Isolation Kit in normal pregnancy, platelet extraction mirVanaTM miRNA Isolation Kit in normal pregnancy were performed. In patients with HELLP syndrome, there were two clinical studies. The patients who had the symptoms of the disease made sure that the patients with the symptoms of the disease were suffering from severe illness, and that the patients with the symptoms of the disease were still suffering from severe illness. There were more than 5 cases of normal pregnancy with pregnancy-induced hypertension syndrome.