Treatment with growth factor prosaposin in a mammalian model of spina bifida

生长因子前塞塞蛋白治疗哺乳动物脊柱裂模型

• 批准号: 21K09324
• 负责人: カーン シャキル
• 金额: $ 2.66万
• 依托单位: Oita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K09324/
• 关键词: Spina Bifida Aperta; Chicken model; Mammalian model; Prosaposin; Neurotrophic factor; Therapeutic potentiality; 二分脊椎; 哺乳類モデル; プロサポシン; 神経成長因子; 治療; ラット; マウス

Spina bifida aperta (SBA), a developmental disorder of the spinal cord, causes high pediatric mortality and is the leading cause of lifelong neurological complications including paralysis, orthopedic abnormalities, problems with bladder and bowel control, and painful complications. Current therapeutics for SBA have various limitations, including incomplete preservation of spinal tissue and the inability to rescue sensorimotor functions. We found that intra-amniotic delivery of prosaposin-derived 18 mer-peptide (PS18), a potential neurotrophic factor, to the chick embryo with SBA enhances the regeneration process and protects spinal tissue in developing spinal cord. Furthermore, treatment with PS18 dramatically improved the walking and bowel movement ability and reduce and painful complications of hatched chicks in postnatal life. The results are published as first author in a highly reputed scientific journal (Khan et al., iScience 2023; IF: 6.107).In order to aim for clinical application of PS18, verification using not only the chicken model but also the mammalian SBA model is necessary. In this regard, we are trying to develop a mice model of SBA by inducing Zika virus which causes neural tube malformations and other birth defects. Zika virus solution (1x10^4 PFU in 200μL PBS) was administered subcutaneously to STAT1 KO mice on the 6th day of pregnancy to induce SBA and/or other defects in the fetus. Once a mice model with SBA or other neurological birth defects is developed, we will evaluate the therapeutic efficacy of PS18 with the aim of writing papers.

开放性脊柱裂（SBA）是一种脊髓发育障碍，导致儿童死亡率很高，是终身神经系统并发症的主要原因，包括瘫痪、骨科畸形、膀胱和肠道控制问题以及疼痛并发症。目前的SBA治疗有各种局限性，包括脊髓组织的不完全保存和无法挽救感觉运动功能。我们发现，羊膜内递送鞘脂激活蛋白原衍生的18聚体肽（PS18），一种潜在的神经营养因子，与SBA的鸡胚增强再生过程，并保护脊髓发育中的脊髓组织。此外，用PS18处理显著改善了孵化小鸡的行走和排便能力，并减少了出生后生活中的疼痛并发症。该结果作为第一作者发表在一个高度知名的科学杂志上（Khan et al.，iScience 2023;如果：为了将PS18应用于临床，不仅需要使用鸡模型，还需要使用哺乳动物SBA模型进行验证。在这方面，我们正试图通过诱导寨卡病毒来开发一种SBA小鼠模型，这种病毒会导致神经管畸形和其他出生缺陷。在妊娠第6天，向STAT 1 KO小鼠皮下给予寨卡病毒溶液（溶于200μL PBS的1 × 104 PFU），以诱导胎儿出现SBA和/或其他缺陷。一旦开发出SBA或其他神经系统出生缺陷的小鼠模型，我们将评估PS18的治疗效果，目的是撰写论文。

项目成果

A comprehensive design for prevention and management of COVID-19 in a tertiary medical institution in Bangladesh

孟加拉国三级医疗机构预防和管理 COVID-19 的综合设计

• DOI: 10.3855/jidc.13729
• 发表时间: 2022
• 期刊: The Journal of Infection in Developing Countries
• 作者: Debnath Chitto Ranjan;Khan Md Saiful Islam;Khan Md Sakirul Islam;Nath Proggananda;Majumder Lakshmi Narayan;Monowar Md. Sayem;Khan Monalisa;Islam Farzana;Nabeka Hiroaki;Shimokawa Tetsuya;Matsuda Seiji;Mahtab Mamun Al;Akbar Sheikh Mohammad Fazle
• 通讯作者: Akbar Sheikh Mohammad Fazle

Effect of lithium chloride on food intake, cloacal temperature, voluntary activity, and crop-emptying rate in chicks

氯化锂对雏鸡摄食量、泄殖腔温度、自主活动和嗉囊排空率的影响

• DOI: 10.1016/j.cbpa.2022.111284
• 发表时间: 2022
• 期刊: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
• 作者: Tachibana Tetsuya;Nakatani Ai;Khan Sakirul;Makino Ryosuke;Cline Mark A.
• 通讯作者: Cline Mark A.

Repurposing NASVAC, a hepatitis B therapeutic vaccine, for pre- and postexposure prophylaxis of SARS-CoV-2 infection

• DOI: 10.21203/rs.3.rs-438628/v1
• 发表时间: 2021-04
• 作者: S. M. Akbar;M. Mahtab;J. Aguilar;M. Uddin;Sakirul Khan;O. Yoshida;E. Pentón;Guillén . Gerardo;Y. Hiasa

Unprecedented rise of monkeypox in Europe and America: Are Asian countries ready for a new outbreak during the ongoing COVID-19 pandemic?

• DOI: 10.7189/jogh.12.03066
• 发表时间: 2022-08-31
• 期刊: JOURNAL OF GLOBAL HEALTH
• 影响因子: 7.2
• 作者: Khan, Sakirul;Akbar, Sheikh Mohammad Fazle;Yahiro, Takaaki;Al Mahtab, Mamun;Kimitsuki, Kazunori;Nishizono, Akira
• 通讯作者: Nishizono, Akira

Prosaposin in the rat oviductal epithelial cells

大鼠输卵管上皮细胞中的前列腺素

• DOI: 10.1007/s00441-020-03339-x
• 发表时间: 2020
• 期刊: Cell and Tissue Research
• 影响因子: 3.6
• 作者: Shimokawa Tetsuya;Nabeka Hiroaki;Khan Sakirul Islam;Yamamiya Kimiko;Doihara Takuya;Kobayashi Naoto;Wakisaka Hiroyuki;Matsuda Seiji
• 通讯作者: Matsuda Seiji