Expression analyses for the molecular physiologically founded progression of somatic embryogenesis in the horticultural model culture Cyclamen persicum

在拟议的项目中，将在基因表达研究的基础上，系统地修改园艺模式物种仙客来体细胞胚胎发生的体外繁殖系统。因此，我们的目标是发展假设的基因表达研究的系统发展的体外繁殖系统的适用性。该项目分为两个部分：在A部分，将使用1216个来自桃树胚性细胞培养物的功能注释转录本开发微阵列。将在标准条件下的合子胚胎和体细胞胚胎发育期间以及在发育畸变率较高或胚胎发生能力丧失的培养物中分析所代表的基因的表达。基于这些数据，将对不同功能基因组的相关性进行推论。在项目B部分，将使用实时聚合酶链式反应详细分析选定的功能基因组的表达。在初步研究的基础上，赤霉素生物合成相关基因被选为第一个要分析的功能群。这些基因的表达将在体外培养测试系统中进行研究。这些数据将被用于系统地修改体外培养条件，这将反过来通过基因表达研究来验证。根据A部分的结果，将相应地对选择的另外两个功能基因组执行这一程序。

In the proposed project the in-vitro propagation system for somatic embryogenesis of the horticultural model species Cyclamen persicum will be systematically modified based on gene expression studies. Herewith, we are aiming to develop hypotheses on the suitability of gene expression studies for systematic development of in-vitro propagation systems in general. The project is divided into two parts: In Part A a microarray will be developed using 1,216 functionally annotated transcripts from embryogenic cell cultures of C. persicum. The expression of the represented genes will be analysed during zygotic and somatic embryo development under standard conditions as well as in cultures exhibiting a high rate of developmental aberrations or loss of embryogenic competence. Based on this data, hypotheses will be deduced on the relevance of different functional gene groups. In project part B the expression of selected functional gene groups will be analysed in detail using real-time PCR. Based on preliminary studies, genes involved in gibberellin biosynthesis have been selected as the first functional group to be analysed. Expression of these genes will be investigated in an in-vitro culture test system. These data will be used for systematic modification of the in-vitro culture conditions, which will in turn be validated by gene expression studies. This procedure will be carried out accordingly for two other functional gene groups to be selected based on the results from part A.