Expression analyses for the molecular physiologically founded progression of somatic embryogenesis in the horticultural model culture Cyclamen persicum

本研究拟在基因表达研究的基础上，对园艺模式植物仙客来体细胞胚胎发生的离体繁殖体系进行系统的改良。因此，我们的目标是发展的适用性的基因表达研究的体外繁殖系统的系统开发的假设一般。该项目分为两个部分：A部分将使用1,216个来自C. persicum.将在标准条件下的合子和体细胞胚胎发育期间以及在表现出高发育畸变率或胚胎发生能力丧失的培养物中分析代表基因的表达。基于这些数据，将推导出不同功能基因组相关性的假设。在项目部分B中，将使用实时PCR详细分析选定功能基因组的表达。在初步研究的基础上，选择了参与赤霉素生物合成的基因作为分析的第一个功能组。将在体外培养试验系统中研究这些基因的表达。这些数据将用于体外培养条件的系统性修改，进而通过基因表达研究进行验证。该程序将根据A部分的结果对另外两个待选择的功能基因组进行相应的操作。

In the proposed project the in-vitro propagation system for somatic embryogenesis of the horticultural model species Cyclamen persicum will be systematically modified based on gene expression studies. Herewith, we are aiming to develop hypotheses on the suitability of gene expression studies for systematic development of in-vitro propagation systems in general. The project is divided into two parts: In Part A a microarray will be developed using 1,216 functionally annotated transcripts from embryogenic cell cultures of C. persicum. The expression of the represented genes will be analysed during zygotic and somatic embryo development under standard conditions as well as in cultures exhibiting a high rate of developmental aberrations or loss of embryogenic competence. Based on this data, hypotheses will be deduced on the relevance of different functional gene groups. In project part B the expression of selected functional gene groups will be analysed in detail using real-time PCR. Based on preliminary studies, genes involved in gibberellin biosynthesis have been selected as the first functional group to be analysed. Expression of these genes will be investigated in an in-vitro culture test system. These data will be used for systematic modification of the in-vitro culture conditions, which will in turn be validated by gene expression studies. This procedure will be carried out accordingly for two other functional gene groups to be selected based on the results from part A.