An integrative approach to disease gene discovery combining genetic variation, gene expression, and epigenetics.
结合遗传变异、基因表达和表观遗传学的疾病基因发现的综合方法。
基本信息
- 批准号:10349878
- 负责人:
- 金额:$ 15.97万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-03-01 至 2024-02-29
- 项目状态:已结题
- 来源:
- 关键词:AddressAwardBiologicalBiological ProcessCellsCharacteristicsChromosome MappingClinical MedicineClinical TreatmentComplexConsensusCoronary arteryDataData AnalysesData SetDevelopmentDiseaseDistantEducational process of instructingEducational workshopEnsureEnvironmentEpigenetic ProcessEtiologyFutureGene ExpressionGeneral HospitalsGenesGeneticGenetic VariationGenotype-Tissue Expression ProjectGoalsGuidelinesHeritabilityHeterogeneityInstitutesLeadershipLinkage DisequilibriumMapsMassachusettsMeasuresMedicalMentorsMentorshipMeta-AnalysisMethodologyMethodsModelingMolecularMultiomic DataParticipantPathway interactionsPhasePhenotypeProteomicsQuantitative Trait LociResearchResearch PersonnelResearch TrainingResourcesRunningSamplingScientistSignal TransductionStatistical MethodsTechnologyTestingTherapeuticTissue SampleTissuesTrainingTranscriptTranslatingTranslationsUntranslated RNAVariantWorkage relatedbasebiobankcell typecohortdata toolsdesigndisorder riskepigenomicsexperiencefunctional genomicsgene discoverygenetic analysisgenetic associationgenetic variantgenome wide association studygenome-widegenome-wide analysisgenomic variationhigh dimensionalityhuman diseaseimprovedinsightlarge scale datamaculametabolomicsmethod developmentmolecular phenotypenovelopen sourcerare variantrepositorysingle-cell RNA sequencingskillssymposiumtooltraittranscriptometranscriptome sequencingtranscriptomicstranslational genetics
项目摘要
PROJECT SUMMARY ABSTRACT
Genome-wide association studies (GWASs) have uncovered hundreds of thousands of disease-associated
genetic variations, but a remarkable disconnect persists between GWAS findings and biological insight required
for clinical treatments and medicine advancement. Pinpointing the functional consequences of variants found in
GWASs is complicated by linkage disequilibrium (LD) and the inability to interpret non-coding variations.
Systematic genetic analysis of high-dimensional molecular and cellular datasets such as transcriptomics,
epigenomics, proteomics, and metabolomics, offers the potential to bridge the gap from complex trait association
to relevant biological processes yet poses unsolved computational and analytical challenges.
The candidate proposes to address major gaps in existing methodologies for mapping the genetic basis of
molecular phenotypes and integrating multi-omics data to improve disease gene discovery by developing a suite
of open-source statistical methods and publicly available analytical resources. The candidate will: 1) develop a
novel scalable statistical method to detect genome-wide expression quantitative trait loci (eQTL) using large-
scale bulk or single-cell RNA sequencing (scRNA-seq) data with an extension for rare variants; 2) assemble and
analyze more than 24 readily available bulk and scRNA-seq data sets for a comprehensive repository containing
cis- and trans-eQTLs of both common and rare variations; 3) develop an integrative method to improve power
for disease gene discovery by combining epigenetics, genome-wide eQTLs, and genetic variations.
The proposed research and training plan were carefully designed to confer expertise in four domains: 1)
transcriptomics and epigenomics, 2) statistical methods development, 3) large-scale data analysis and tools,
and 4) professional development. These skills are fundamental to the candidate’s goal of becoming a leading
investigator who develops and applies statistical methods to understand molecular mechanisms of complex
diseases and traits. In addition to research training, the candidate will take coursework to gain greater expertise
in transcriptomics and functional genomics, participate in regular seminars, attend workshops and conferences,
and gain mentorship and teaching experience. All research will be conducted in the Analytic and Translational
Genetics Unit at Massachusetts General Hospital and the Broad Institute with mentorship from renowned
scientists Drs. Benjamin Neale and Mark Daly. Additional guidance from leading experts Drs. Xihong Lin, Ramnik
Xavier, Kristin Ardlie, and Bradley Bernstein will ensure exceptional guidance and support. Overall, the training
environment is outstanding, the mentors and advisors are world-class, the proposed studies address an urgent
unmet need, and the additional skills gained in this award will poise the candidate to establish independent
leadership in leveraging statistical genetics and large-scale multi-omics data for disentangling the etiology of
complex diseases.
项目总结摘要
全基因组关联研究(GWAS)已经发现了数十万与疾病相关的
基因变异,但GWAS的发现和所需的生物学洞察力之间仍然存在显著的脱节
用于临床治疗和医学进步。中发现的变体的功能后果
GWASS因连锁不平衡(LD)和无法解释非编码变异而变得复杂。
对高维分子和细胞数据集的系统遗传分析,如转录组学,
表观基因组学、蛋白质组学和代谢组学为弥合复杂性状关联之间的差距提供了可能
对相关生物过程的研究还带来了尚未解决的计算和分析挑战。
候选人建议解决现有方法中存在的重大差距,以绘制出人类遗传基础图
分子表型和整合多组学数据通过开发一套套件来改进疾病基因发现
开放源码统计方法和公开可用的分析资源。候选人将:1)制定一项
一种新的可扩展统计方法检测全基因组表达的数量性状座位(EQTL)
大规模批量或单细胞RNA测序(scRNA-seq)数据,包括稀有变异的扩展;2)组装和
分析超过24个现成的批量和scRNA-seq数据集,以用于包含以下内容的综合存储库
常见和罕见变异的顺式和反式eQTL;3)开发一种综合方法来提高动力
通过结合表观遗传学、全基因组eQTL和遗传变异来发现疾病基因。
拟议的研究和培训计划经过精心设计,以提供四个领域的专门知识:1)
转录学和表观基因组学,2)统计方法的发展,3)大规模数据分析和工具,
4)职业发展。这些技能是候选人实现成为领导者的目标的基础
研究人员,开发并应用统计学方法来理解复杂分子机制
疾病和特征。除了研究培训,应聘者还将参加课程学习以获得更多的专业知识
在转录学和功能基因组学方面,参加定期研讨会,参加研讨会和会议,
并获得导师和教学经验。所有研究将在分析和翻译中进行
马萨诸塞州总医院和布罗德研究所的遗传学部门,由著名的
科学家本杰明·尼尔博士和马克·戴利博士。来自顶尖专家林锡鸿博士的补充指导
泽维尔、克里斯汀·阿德利和布拉德利·伯恩斯坦将确保获得特殊的指导和支持。总体而言,培训
环境出众,导师和顾问都是世界一流的,建议研究解决一个紧迫的问题
未得到满足的需求,以及在这个奖项中获得的额外技能将使候选人保持平衡,建立独立
在利用统计遗传学和大规模多组学数据解开糖尿病病因方面的领先地位
复杂的疾病。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Wei Zhou其他文献
Spin Waves Excitations of Co/PtMultilayers
Co/Pt 多层膜的自旋波激发
- DOI:
- 发表时间:
2012 - 期刊:
- 影响因子:0
- 作者:
Wei Zhou - 通讯作者:
Wei Zhou
Wei Zhou的其他文献
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{{ truncateString('Wei Zhou', 18)}}的其他基金
Targeting LKB1-null lung adenocarcinoma with innate immune system
利用先天免疫系统靶向 LKB1 缺失的肺腺癌
- 批准号:
10752833 - 财政年份:2023
- 资助金额:
$ 15.97万 - 项目类别:
An integrative approach to disease gene discovery combining genetic variation, gene expression, and epigenetics.
结合遗传变异、基因表达和表观遗传学的疾病基因发现的综合方法。
- 批准号:
10581608 - 财政年份:2022
- 资助金额:
$ 15.97万 - 项目类别:
Role of orexin/hypocretin circuit in anesthesia and analgesia
食欲素/下丘脑分泌素回路在麻醉和镇痛中的作用
- 批准号:
10651642 - 财政年份:2020
- 资助金额:
$ 15.97万 - 项目类别:
Role of orexin/hypocretin circuit in anesthesia and analgesia
食欲素/下丘脑分泌素回路在麻醉和镇痛中的作用
- 批准号:
10186780 - 财政年份:2020
- 资助金额:
$ 15.97万 - 项目类别:
Role of orexin/hypocretin circuit in anesthesia and analgesia
食欲素/下丘脑分泌素回路在麻醉和镇痛中的作用
- 批准号:
10430182 - 财政年份:2020
- 资助金额:
$ 15.97万 - 项目类别:
Role of orexin/hypocretin circuit in anesthesia and analgesia
食欲素/下丘脑分泌素回路在麻醉和镇痛中的作用
- 批准号:
10040369 - 财政年份:2020
- 资助金额:
$ 15.97万 - 项目类别:
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