Trans-omics analysis of the difference between Cortical and Trabecular bone.

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In this study, a bioinformatic methodology employing trans-omics analysis and the Matrisome database is being employed to investigate the differences in extracellular matrix composition and the associated regulatory networks between cortical and trabecular bone.During this period, FASTQ files analysis workflow was stablished and Gene Read Counts trials were performed. RNA sequencing environment for Weighted gene co-expression network analysis setting was completed to identify modules or groups of genes that exhibit similar expression patterns across different samples. This method assigns weights to gene-gene connections based on the strength of their co-expression relationships, allowing the identification of highly interconnected gene modules and the functional relationships and regulatory mechanisms underlying biological processes by identifying key genes within modules and elucidating their potential roles in specific biological pathways or diseases.DESeq2/Limma for fold change and gene list environment was set for Pathway analysis to perform enrichment analysis and visualize the results, to gain insights into the bone biological systems and to identify potential targets for therapeutic intervention.