权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Fundamental research of antitumor activity of arsenic compound in combination with active bufadienolide compounds against glioblastoma cells

砷化合物联合活性蟾蜍二烯内酯化合物抗胶质母细胞瘤细胞活性的基础研究

基本信息

批准号：
20K07136
负责人：
袁博
金额：
$ 2.75万
依托单位：
Josai University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2020
资助国家：
日本
起止时间：
2020-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

本年度に、膠芽腫細胞U-87に対する亜ヒ酸ナトリウム（AsIII）、ブファジェノライドのarenobufagin (Areno)およびgamabufotalin (Gama)の併用効果を以下のように比較検討した。①AsIII、ArenoあるいはGamaのそれぞれは、濃度依存的にU-87細胞の細胞増殖を有意に抑制した。また、それぞれの単独処理より、AsIIIとArenoあるいはGamaの併用は、相乗的にU-87細胞の増殖を抑制した。また、Gamaと比較したところ、Arenoの方がより効率よくAsIIIの増殖抑制効果を増強した。細胞内ヒ素の蓄積量の変動を検討したところ、興味深いことに、Gamaと比較しArenoの共存下は有意に細胞内ヒ素の蓄積量を高めたことから、Arenoがヒ素の細胞内への取り込みに促進することが考えられた。Arenoがヒ素による細胞毒性に関わるトランスポーターへの影響を引き続き検証する。②AsIIIとArenoの併用での処理を行い、細胞溶解液中のcaspase-8, -9および-3の活性を測定したところ、caspase-8より、caspase-9および-3の活性はより強く誘導されたことから、caspase-9, -3が関わるintrinsic pathwayが薬物に誘導されるアポトーシスに強く関与することが示唆されたほか、ネクローシスの関与も考えられた。③DNAダメージマーカーであるγH2AXの誘導が観察されたことから、薬物に起因するDNAダメージがG2/Mアレストの誘導に寄与することが考えらえれた。④オートファジー細胞死およびNotchシグナリングへの阻害は、薬物の併用効果に大きく関与することが示唆された。

This year, we will compare the results of arenobufagin (Areno), gamabufotalin (Gama), and so on, in terms of the following results. The colonies of 1AsIII, Areno and Gama cells were suppressed intentionally, and the degree-dependent cell colonies were intentionally inhibited. It was found that there was no significant difference between the control group and the control group, and there was no significant difference between the control group and the control group. The results showed that there was no significant difference between the control group and the control group, and there was no significant difference between the control group and the control group. The results showed that there was no significant difference between the control group and the control group. There was no significant difference between the two groups. The results of AsIII colonization inhibition were significantly stronger than those of Gama and AsIII. In the presence of Gama, Areno, Areno and Areno, the accumulation of intracellular hormone is more active than that of high concentration of intracellular hormone, and the activity of intracellular hormone is increased. Areno, cytotoxicity, cytotoxicity. 2AsIII Areno was used to determine the activity of caspase-8, caspase-8, caspase-9 and caspase-9 in cytosol solution. -3. Please tell me that you need to know that you are not going to have a problem with the intrinsic pathway. 3DNA, please contact me. I need to know that the cause of the problem is that the cause of the problem is the cause of the problem. I am sorry to know that the cause of the problem has been sent to you by sending it to you. 4. Please tell me that the cell is dead, that the Notch is dead, that the nuisance is blocked, and that the object is instigated by the use of fruit and fruit.