Reactivation of Tumor-suppressor MicroRNAs in Cancer Cells by Epigenome Editing

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The objective of this project was to develop a reliable system for targeted demethylation and reactivation of specific cancer-related microRNAs (miRNAs). By adapting the CRISPR-Cas9 system and establishing an innovative editing technology, we have been able to accurately manipulate the epigenome of tumor-suppressor miRNAs for functional analyses and potential therapeutic applications. Thus far, the following aims have been achieved:1. Targeted demethylation of the tumor-suppressor miR-122 using the dCas9-TET1 epigenome editing system2. Identification of miR-122 targets using miRNA epigenome editing3. miR-122 editing negatively impacts hepatic cancer cell growth through SLC2A3 targeting