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Programmable control over histone acetylation at human regulatory elements using precision epigenome editing

使用精确表观基因组编辑对人类调控元件的组蛋白乙酰化进行可编程控制

基本信息

批准号：
10669331
负责人：
Isaac Hilton
金额：
$ 56.88万
依托单位：
RICE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-09-01 至 2024-02-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10669331
关键词：
Acetylation Architecture Automobile Driving Biological Phenomena CRISPR screen CRISPR/Cas technology Cell Nucleus Cells Chimeric Proteins Chromatin Chromatin Loop Clinical Trials Clustered Regularly Interspaced Short Palindromic Repeats Code Complex DNA Data Deacetylase Deacetylation Disease Dissection Engineering Enhancers Epigenetic Process Future Gene Activation Gene Expression Gene Expression Profile Gene Expression Regulation Genetic Transcription Genome Genomics Global Change Goals Health Histone Acetylation Histone Deacetylase Histone Deacetylation Histones Human Human Activities Human Genome In Vitro Knock-out Light Link Location Lysine Malignant Neoplasms Mass Spectrum Analysis Mutation Nucleosomes Outcome Pathologic Pathology Patients Pattern Pharmaceutical Preparations Play Post Translational Modification Analysis Post-Translational Protein Processing Process Production Proteins RNA Regulator Genes Regulatory Element Role Signal Transduction Source System Technology Testing Therapeutic Transcription Coactivator Untranslated RNA Variant Vorinostat Work Writing base cell type combinatorial cytotoxic developmental disease epigenetic therapy epigenome editing epigenomics experience experimental study genome-wide histone acetyltransferase human disease improved inhibitor innovation insight nervous system disorder new technology nuclease prevent programs promoter protein complex scaffold screening selective expression side effect small molecule stem tool virtual

项目摘要

PROJECT SUMMARY/ABSTRACT Dysregulated gene expression contributes to nearly every human disease. The level at which we understand the inherent complexity of gene regulation ultimately dictates our ability to link and/or manipulate biomolecular changes and pathology. Beyond their function as a structural substrate, histone proteins play key roles in gene expression by selectively gating access to DNA and thereby guiding when and how transcriptional machinery engages the human genome. Histone-based gene regulatory control largely stems from post-translational modifications (PTMs) to histone proteins themselves. One type of PTM, histone lysine acetylation, is particularly critical for gene regulation and human health, because overall acetylation levels tightly correlate with genomic activity and gene expression, and inappropriate histone acetylation patterns are linked to diverse human diseases. In fact, small molecules that globally change histone acetylation across the human genome have emerged as important therapeutics. However, in many patients these drugs can be ineffective and/or can result in toxic side effects. Furthermore, small molecules that globally alter histone acetylation patterns cannot be used to dissect how changes at specific locations in the human genome drive disease pathology. Robust tools that enable precise and targeted control over endogenous histone acetylation are urgently needed, because these technologies could illuminate the function(s) that this complex epigenomic signature plays within the human genome and open the door to new sophisticated epigenetic therapies. In this project, we will fulfill this urgent need by building synthetic and precisely targetable biomolecules that mimic the spectrum of activities displayed by natural human histone acetyltransferases (HATs) and histone deacetylases (HDACs) (Aim 1). Specifically, we will combine the programmability of the nuclease null CRISPR/Cas9 (dCas9) scaffold with different classes of human HATs/HDACs and we will use these technologies to probe the selectivity of different HATs/HDACs in vitro and within native human chromatin. We will integrate our results with epigenomic profiling data to define how epigenetic marks, nucleosome occupancy, and cis regulatory element proximity influence the effects of histone acetylation at human enhancers and promoters. We will also use mass spectrometry, dCas9-based transcriptional activators and HATs, and genome-scale knockout screening to define the proteins/protein complexes that support histone acetylation-based gene activation (Aim 2). Finally, we will establish the impact of precisely targeted histone acetylation/deacetylation on enhancer activity and enhancer-promoter interactions (Aim 3). Experiments will be conducted at testbed human loci that have broad significance to human health and mechanistic epigenetics, and that will serve as proof-of-principle for interrogating virtually any regulatory element or locus in the human genome in future efforts. To accomplish the aims of this project, we have assembled an experienced team with synergistic expertise in CRISPR/Cas- based epigenome editing, histone PTM analysis, and functional dissection of enhancer activity.

项目总结/文摘