Mitochondrial and cytosolic maturation of human FeS-dependent molybdenum cofactor synthesis MOCS1A proteins and links between both cofactor pathways

人 FeS 依赖性钼辅因子合成 MOCS1A 蛋白的线粒体和细胞质成熟以及两条辅因子途径之间的联系

• 批准号: 311772630
• 负责人: Professor Dr. Günter Schwarz
• 金额: --
• 依托单位: Institut für Biochemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/311772630?language=en
• 关键词: Mitochondrial; cytosolic; maturation; human; FeS

Molybdenum (Mo)-containing enzymes catalyze key reactions in the global cycles of life. They are dependent on the biosynthesis of the Mo cofactor (Moco) as well as FeS clusters, which are required in the first step of Moco synthesis, the conversion of GTP into cyclic pyranopterin monophosphate (cPMP) and function as cofactors in Mo enzymes. A defect in the biosynthesis of Moco leads to a severe form of inborn error in metabolism, mainly caused by the loss of sulfite oxidase activity characterized by rapidly progressing neurodegeneration and childhood death. We have developed a first therapy for patients with mutations in the MOCS1 gene that produces alternatively spliced transcripts encoding for two proteins. MOCS1A requires two [4Fe-4S] clusters and belongs to the superfamily of radical SAM enzymes. The second translation product represents the MOCS1AB protein, with an inactive MOCS1A domain and a C-terminal MOCS1B domain with catalytic activity. During the previous funding period we were able to demonstrate cytosolic localization of two MOCS1A splice variants, while the two other MOCS1A splice variants, as well as all MOCS1AB splice variants localized to mitochondria. Furthermore and surprisingly, we found during the import process that MOCS1AB is proteolytically cleaved resulting in a small MOCS1B protein, which localizes similar to MOCS1A to the mitochondrial matrix. In vivo activity of MOCS1B could be confirmed by a patient case investigated within the SPP, where a homozygous frameshift mutation resulted in separate expression of MOCS1B. In the following funding period we aim to address two key aspects unresolved in human Moco synthesis and Moco deficiency (MoCD). First, we plan to investigate the differences of cytosolic and mitochondrial MOCS1A proteins, given that both classes of proteins are expressed under physiological conditions and have been shown to play a role in Moco biosynthesis. Therefore we will biochemically characterize different MOCS1A variants including the determination of kinetic parameters, based on our established anaerobic purification and cPMP in vitro synthesis protocols. This will also include crystallization of MOCS1A (O. Einsle), as well as EPR- (with A. Pierik) and Mössbauer-spectroscopy (V. Schünemann) of cytosolic and mitochondrial MOCS1A proteins purified from Sf9-insect cell culture. In the second part of this project we investigate the cellular and homeostatic link between FeS cluster biogenesis and Moco synthesis. We aim to understand the impact of impairments of iron homeostasis and FeS-cluster biogenesis on Moco biosynthesis via monitoring Moco-enzyme activities and Moco biosynthesis in cell culture, mouse lines and patient samples in collaboration with different groups of the SPP (R. Lill, C. Berndt, A. Steinbicker). Vice versa, we will probe how alterations in sulfite oxidase activity impact FeS-cluster biogenesis in a novel sulfite oxidase deficient mouse line, which has been created in our lab.

含钼酶在全球生命周期中催化关键反应。它们依赖于钼辅因子(MOCO)和FeS簇的生物合成，这是MOCO合成的第一步所必需的，GTP转化为环状吡喃喋呤单磷酸(CPMP)，并在钼酶中作为辅因子发挥作用。MOCO的生物合成缺陷会导致一种严重的先天性代谢错误，主要是由于亚硫酸盐氧化酶活性的丧失，其特征是快速进展的神经退化和儿童死亡。我们已经为MOCS1基因突变的患者开发了第一种治疗方法，这种方法可以产生编码两种蛋白质的选择性剪接转录本。MOCS1A需要两个[4Fe-4S]簇，属于自由基SAM酶超家族。第二个翻译产物代表MOCS1AB蛋白，具有非活性的MOCS1A结构域和具有催化活性的C-末端MOCS1B结构域。在之前的资助期间，我们能够证明两个MOCS1A剪接变体的胞浆定位，而另外两个MOCS1A剪接变体以及所有MOCS1AB剪接变体定位于线粒体。此外，令人惊讶的是，我们在进口过程中发现MOCS1AB被蛋白水解性切割，产生了一个小的MOCS1B蛋白，它定位于线粒体基质中与MOCS1A类似的位置。在SPP中调查的一个患者病例可以证实MOCS1B的体内活性，在该病例中，纯合移码突变导致MOCS1B单独表达。在接下来的资助期间，我们的目标是解决人类Moco合成和Moco缺乏症(MoCD)中尚未解决的两个关键问题。首先，我们计划研究胞质和线粒体MOCS1A蛋白的差异，因为这两类蛋白都在生理条件下表达，并已被证明在MOCO生物合成中发挥作用。因此，我们将在我们建立的厌氧净化和cPMP体外合成方案的基础上，对不同的MOCS1A变异体进行生化表征，包括动力学参数的确定。这也将包括MOCS1A的结晶(O.Einsle)，以及从Sf9昆虫细胞培养中纯化的胞浆和线粒体MOCS1A蛋白的EPR-(与A.Pierik)和穆斯堡尔谱(V.Schünemann)。在这个项目的第二部分，我们研究了FeS簇生物发生和MOCO合成之间的细胞和动态平衡联系。我们的目标是通过与SPP的不同小组(R.Lill，C.Berndt，A.Steinbicker)合作，监测细胞培养、小鼠细胞系和患者样本中的MOCO酶活性和MOCO生物合成，了解铁稳态和FeS簇生物发生的损害对MOCO生物合成的影响。反之亦然，我们将探索亚硫酸盐氧化酶活性的变化如何影响我们实验室创造的一种新的亚硫酸盐氧化酶缺陷小鼠系的FES簇的生物发生。