Single-cell transcriptomic and epigenomic analysis of young and elder mouse gastric mucosa infected by Helicobacter pylori

幽门螺杆菌感染的幼年和老年小鼠胃粘膜的单细胞转录组和表观基因组分析

• 批准号: 20K16347
• 负责人: Liu Yuyu
• 金额: $ 2.33万
• 依托单位: Hoshi University (2022)National Cancer Center Japan (2020-2021)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K16347/
• 关键词: Gastric mucosa; Gastric stem cells; Aging; Epigenetics; Chromatin accessibility; Helicobacter pylori; DNA methylation; Gastric cancer; Single-cell analysis; Stem cell epigenetics

This year, we conducted and analyzed scATAC-seq data obtained from gastric mucosa of 3-week-old and 20-week-old mice. The results indicate that there are differences in chromatin accessibility in gastric epithelial cells, particularly in stem cells, between the two age groups, while the expression profiles are similar. Additionally, the chromatin accessibility in fibroblasts remained consistent with age, but the expression profiles differed.Moreover, we established organoids from single pyloric glands and conducted Wnt withdrawal experiment again. Organoids derived from single glands of 3-week-old mice antrum tissue exhibited a more pronunced Wnt-dependency than those derived from bulk glands. In contrast, organoids derived from 20-week-old mice were unaffected by Wnt withdrawal.

今年，我们对从 3 周龄和 20 周龄小鼠胃粘膜获得的 scATAC-seq 数据进行了分析。结果表明，两个年龄组之间胃上皮细胞，特别是干细胞的染色质可及性存在差异，但表达谱相似。此外，成纤维细胞的染色质可及性与年龄保持一致，但表达谱有所不同。此外，我们从单个幽门腺建立了类器官，并再次进行了Wnt撤回实验。源自 3 周龄小鼠胃窦组织单个腺体的类器官比源自大块腺体的类器官表现出更明显的 Wnt 依赖性。相比之下，来自 20 周龄小鼠的类器官则不受 Wnt 撤除的影响。

项目成果

Comparative analysis of epithelial and stromal responses to H. pylori infection in infant and young adult mice

婴儿和年轻成年小鼠上皮和基质对幽门螺杆菌感染反应的比较分析

• 发表时间: 2021
• 作者: Okazaki Keito;Anzawa Hayato;Liu Zun;Ota Nao;Kitamura Hiroshi;Onodera Yoshiaki;Alam Md. Morshedul;Matsumaru Daisuke;et al.;Yu-Yu Liu
• 通讯作者: Yu-Yu Liu

Plastic stromal-epithelial crosstalk in infant mouse stomach may underlie aggressive phenotypes in AYA cancers.

幼年小鼠胃中的塑料基质-上皮串扰可能是 AYA 癌症侵袭性表型的基础。

• 发表时间: 2022
• 作者: Yuyu Liu;Naoko Hattori;Chihiro Takeuchi;Satoshi Yamashita and Toshikazu Ushijima.
• 通讯作者: Satoshi Yamashita and Toshikazu Ushijima.

Single-nucleus RNA-seq of an RNase-rich tissue, and age-specific cell population profiles in mouse stomach.

富含 RNase 的组织的单核 RNA 测序，以及小鼠胃中年龄特异性细胞群的概况。

• 发表时间: 2022
• 作者: YuYu Liu;Naoko Hattori;Chihiro Takeuchi;Satoshi Yamashita and Toshikazu Ushijima.
• 通讯作者: Satoshi Yamashita and Toshikazu Ushijima.

CUT&RUN method identifies histone modifications and bivalent chromatin at single nucleosome resolution

切

• 发表时间: 2021
• 作者: 近藤慎吾;奥田賢;齋藤梨帆;加藤優;杉本芳一;Yu-Yu Liu
• 通讯作者: Yu-Yu Liu