Single-cell transcriptomic and epigenomic analysis of young and elder mouse gastric mucosa infected by Helicobacter pylori

今年，我们进行并分析了从3周龄和20周龄小鼠的胃粘膜获得的SCATAC-SEQ数据。结果表明，两个年龄段的胃上皮细胞，尤其是干细胞，在染色质可及性方面存在差异，但表达谱相似。此外，成纤维细胞中染色质的可及性与年龄保持一致，但表达谱不同。此外，我们从单个幽门腺中建立了有机体，并再次进行了Wnt退出实验。从3周龄小鼠胃窦组织的单腺体中提取的有机化合物比从大体积腺体中提取的有机化合物表现出更明显的Wnt依赖性。相比之下，来自20周龄小鼠的有机类化合物不受WNT停用的影响。

This year, we conducted and analyzed scATAC-seq data obtained from gastric mucosa of 3-week-old and 20-week-old mice. The results indicate that there are differences in chromatin accessibility in gastric epithelial cells, particularly in stem cells, between the two age groups, while the expression profiles are similar. Additionally, the chromatin accessibility in fibroblasts remained consistent with age, but the expression profiles differed.Moreover, we established organoids from single pyloric glands and conducted Wnt withdrawal experiment again. Organoids derived from single glands of 3-week-old mice antrum tissue exhibited a more pronunced Wnt-dependency than those derived from bulk glands. In contrast, organoids derived from 20-week-old mice were unaffected by Wnt withdrawal.