Single-cell transcriptomic and epigenomic analysis of young and elder mouse gastric mucosa infected by Helicobacter pylori

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This year, we conducted and analyzed scATAC-seq data obtained from gastric mucosa of 3-week-old and 20-week-old mice. The results indicate that there are differences in chromatin accessibility in gastric epithelial cells, particularly in stem cells, between the two age groups, while the expression profiles are similar. Additionally, the chromatin accessibility in fibroblasts remained consistent with age, but the expression profiles differed.Moreover, we established organoids from single pyloric glands and conducted Wnt withdrawal experiment again. Organoids derived from single glands of 3-week-old mice antrum tissue exhibited a more pronunced Wnt-dependency than those derived from bulk glands. In contrast, organoids derived from 20-week-old mice were unaffected by Wnt withdrawal.