权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The role of myoinhibitory peptides (MIPs) in the circadian system of the Madeira cockroach Rhyparobia maderae

肌抑制肽（MIP）在马德拉蟑螂 Rhyparobia maderae 昼夜节律系统中的作用

基本信息

批准号：
315228632
负责人：
Professorin Dr. Monika Stengl
金额：
--
依托单位：
Abteilung Tierphysiologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2016
资助国家：
德国
起止时间：
2015-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/315228632?language=en
关键词：
role myoinhibitory peptides MIPs circadian

项目摘要

The Madeira cockroach Rhyparobia maderae is an established model organism for circadian rhythm research. We identified the accessory medulla (AME) with associated pigment-dispersing factor-immunoreactive (PDF-ir) neurons as circadian pacemaker center controlling circadian locomotor activity rhythms. The AME neurons are abundant in partly colocalized neuropeptides. For example, PDF and myoinhibitory peptide (MIP) are colocalized in two neurons. The function of MIP in the cockroach clock is not known. In Drosophila melanogaster the sex peptide receptor (SPR) is a MIP receptor which controls a physiological/behavioral switch from the non-mated to the mated state. With transcriptome analysis we previously identified a SPR candidate in R. maderae. Using heterologous expression studies combined with BRET assays we want to examine its function. In Ca2+-imaging assays combined with immunocytochemistry, with application of MIPs to primary cell cultures of the AME we will characterize MIP signaling in the cockroach clock. We want to determine signal transduction cascades of MIP in the circadian clock and identify MIP-sensitive AME cells. Furthermore, in behavioral assays combined with immunocytochemistry we will examine whether MIP-signaling and mating/post-mating behaviors regulate each other. We expect to gain insights into specific functions of MIPs and into general principles of neuropeptide function in the circadian system and in insect brains in general.

马德拉蟑螂（Rhyparobia maderae）是昼夜节律研究的一种已建立的模式生物。我们发现副髓质（AME）与相关的色素分散因子免疫反应（PDF-ir）神经元是控制昼夜运动活动节律的昼夜节律起搏器中心。AME神经元具有丰富的部分共定位神经肽。例如，PDF和肌抑制肽（MIP）在两个神经元中共定位。MIP在蟑螂生物钟中的作用尚不清楚。在黑腹果蝇中，性肽受体（SPR）是一种MIP受体，控制着从非交配状态到交配状态的生理/行为转换。通过转录组分析，我们之前在马德龙中发现了一个SPR候选基因。利用异源表达研究结合BRET检测，我们想要检验它的功能。在结合免疫细胞化学的Ca2+成像分析中，将MIP应用于AME的原代细胞培养中，我们将表征蟑螂时钟中的MIP信号。我们想要确定MIP在生物钟中的信号转导级联，并识别MIP敏感的AME细胞。此外，在结合免疫细胞化学的行为分析中，我们将检查mip信号和交配/交配后行为是否相互调节。我们希望深入了解MIPs的具体功能，以及昼夜节律系统和昆虫大脑中神经肽功能的一般原理。