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広汎性脱分極 (皮質拡延性抑制)が脳虚血に及ぼす影響についての研究

广泛去极化（皮质扩散抑制）对脑缺血影响的研究

基本信息

批准号：
20K17970
负责人：
杉本至健
金额：
$ 2.75万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Early-Career Scientists
财政年份：
2020
资助国家：
日本
起止时间：
2020-04-01 至 2024-03-31
项目状态：
已结题

项目摘要

CSDは脳梗塞巣を増大させる。この仮説は、脳梗塞体積とCSDの出現数が相関することと、人為的に誘発したCSDが脳梗塞巣を増大したという２つの実験結果に由来する。しかしながら、塩化カリウム滴下、電気刺激、Pinprick、エンドセリンの脳表への滴下などこれまで報告されているCSD誘発方法はそれ自体が侵襲的であり、組織損傷を来す。本研究はOptogeneticsで非侵襲的に誘発したCSDが脳虚血において保護的に働くか傷害的に働くかを明らかにすることを目的とする。本目的を達成するためにまずOptogenetic spreading depolarizationが脳虚血に及ぼす影響を検討した。すなわち神経細胞にchannelrhodopsin-2（ChR2）を発現したトランスジェニックマウス（Optogeneticマウス）にMicrovascular clipを用いて脳梗塞を作成し、1時間の脳虚血中にIntactな頭蓋骨の上から光刺激（optogenetic stimulation (470 nm, 1-2 mw, 10秒) を行い、繰り返しOptogenetic CSDを誘発させた。48時間後の脳梗塞体積を、CSDを追加（誘発）しなかった対照群と比較し、CSDが脳梗塞に及ぼす影響を検討した。1時間の虚血の間にOptogenetic CSD群は対照群と比較し5倍のCSDを生じた。しかしながら、Optogenetic CSD群の48時間後の脳梗塞体積は対照群と比較し有意差を認めず、むしろ小さくなる傾向を認めた。Optogeneticsで非侵襲的に誘発したCSDは脳虚血を増悪させなかった。これまでのCSD誘発方法自体が脳虚血に対し有害であった可能性がある。

The CSD is clogged with a large infarct. In recent years, the CSD of the infarct and the infarct have caused significant changes in the number of patients, the number of people who have suffered from each other, and the number of people who have suffered from the same disease. The CSD has caused a great deal of trouble. This is due to the use of electronic stimuli, Pinprick, electrical stimulation, electronic stimulation, electrical stimulation, electronic stimulation, electronic stimulation, In this study, Optogenetics non-invasive drugs, such as CSD, deficiency blood, protection, protection, damage, damage, prevention and treatment, were used in this study. in this study, the purpose of this study was not to be intrusive. The purpose of this article is to make sure that the deficiency of blood and the shadow of blood deficiency are caused by Optogenetic spreading depolarization. Cell cycle channelrhodopsin-2 (ChR2) showed that the Microvascular clip was made with infarct (Optogenetic), and light stimulation (optogenetic stimulation (470 nm, 1-2 mw, 10 seconds) was performed on the bone of the Intact in the empty blood for 1 time, and the Optogenetic CSD was returned. 48 hours later, the infarct body was active, and CSD was added to compare the infarct body, CSD infarct and infarct volume. In 1 time, the number of Optogenetic CSD group photos was 5 times higher than that of CSD group photos. After 48 hours in the Optogenetic CSD group and the infarct group, the infarct body photo group was more sensitive than the intended one, and the infarct group was more sensitive than the others. Optogenetics's non-invasive drugs, such as CSD, deficiency of blood, blood deficiency, blood deficiency and blood deficiency. The method of autogenesis, deficiency of blood, harmfulness, and possibility of CSD infection.