Development of a dual-mode optical/microgravimetric biosensor for the detection of three prostate cancer biomarkers

开发用于检测三种前列腺癌生物标志物的双模式光学/微重力生物传感器

• 批准号: 20K20237
• 负责人: Funari Riccardo
• 金额: $ 2.58万
• 依托单位: Okinawa Institute of Science and Technology Graduate University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Early-Career Scientists
• 财政年份: 2020
• 资助国家: 日本
• 起止时间: 2020-04-01 至 2021-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20K20237/
• 关键词: Biosensor; Prostate cancer; SARS-CoV-2; Cancer biomarkers; dual-mode sensing; Antibody

I was able to develop an opto-microfluidic sensing platform to detect specific biomarkers in human plasma. The ongoing global pandemic of SARS-CoV-2 motivated us to use this device to detect first COVID-19 related proteins such as anti-SARS-CoV-2 S antibodies (DOI: 10.1016/j.bios.2020.112578).This device has been further developed to achieve the detection of multiple biomarkers in the same sample using a single integrated device, and is the subject of a recently submitted pubblication about COVID-19 reasarch and of a next paper on prostate cancer biomarkers detection in human plasma. Specifically, this biosensor allows to detect prostate specific antigen, matrix metalloproteinase-2 and anti-alpha-methylacyl-CoA in real sample with high sensitivity (LOD < 0.5 ng/mL) in less than 30 min.

我能够开发一个光微流体传感平台来检测人类血浆中的特定生物标志物。SARS-CoV-2的全球大流行促使我们使用该设备来检测第一个COVID-19相关蛋白，如抗SARS-CoV-2 S抗体（DOI：10.1016/j.bios.2020.112578）。该设备已进一步开发，以实现使用单个集成设备检测同一样本中的多种生物标志物，并且是最近提交的关于COVID-19研究的出版物和关于人血浆中前列腺癌生物标志物检测的下一篇论文的主题。具体地，该生物传感器允许在不到30分钟内以高灵敏度（LOD < 0.5 ng/mL）检测真实的样品中的前列腺特异性抗原、基质金属蛋白酶-2和抗-α-甲基酰基-CoA。

项目成果

Metal-Enhanced Fluorescence Immunosensor Based on Plasmonic Arrays of Gold Nanoislands on an Etched Glass Substrate

• DOI: 10.1021/acsanm.0c02388
• 发表时间: 2020-09
• 期刊: ACS Applied Nano Materials
• 影响因子: 5.9
• 作者: Bruno Miranda;K. Chu;P. Maffettone;A. Shen;R. Funari

Low-cost chip for detecting COVID-19 antibodies

用于检测 COVID-19 抗体的低成本芯片

Detection of antibodies against SARS-CoV-2 spike protein by gold nanospikes in an opto-microfluidic chip

• DOI: 10.1016/j.bios.2020.112578
• 发表时间: 2020-12-01
• 期刊: BIOSENSORS & BIOELECTRONICS
• 影响因子: 12.6
• 作者: Funari, Riccardo;Chu, Kang-Yu;Shen, Amy Q.
• 通讯作者: Shen, Amy Q.